Mechanical Stress-Dependent Autophagy Component Release via Extracellular Nanovesicles in Tumor Cells
文献类型：期刊论文
| 作者 | Wang, KZ; Wei, YH; Liu, WJ; Liu, L; Guo, Z; Fan, CH; Wang, LH; Hu, J; Li, B |
| 刊名 | ACS NANO
 |
| 出版日期 | 2019 |
| 卷号 | 13期号:4页码:4589—4602 |
| ISSN号 | 1936-0851 |
| 关键词 | STARVATION-INDUCED AUTOPHAGY TRANSFERRIN RECEPTOR BIOLOGICAL FUNCTIONS MEMBRANE-VESICLES CANCER CELLS PROTEINS FUSION DEGRADATION INHIBITION GROWTH |
| DOI | 10.1021/acsnano.9b00587 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Tumor cells metastasizing through the bloodstream or lymphatic systems must withstand acute shear stress (ASS). Autophagy is a cell survival mechanism that functions in response to stressful conditions, but also contributes to cell death or apoptosis. We predicted that a compensation pathway to autophagy exists in tumor cells subjected to mechanical stress. We found that ASS promoted autophagosome (AP) accumulation and induced release of extracellular nanovesicles (EVs) containing autophagy components. Furthermore, we found that ASS promoted autophagic vesicles fused with multivesicular body (MVB) to form an AP-MVB compartment and then induced autophagy component release into the extracellular space via EVs through the autophagy-MVB-exosome pathway. More importantly, either increasing intracellular autophagosome accumulation or inhibiting autophagic degradation promoted AP-MVB accumulation but did not induce autophagy-associated protein release via EVs except under ASS, demonstrating the existence of a mechanical stress dependent compensation pathway. Together, these findings revealed that EVs provide an additional protection mechanism for tumor cells and counteract autophagy to maintain cellular homeostasis under acute shear stress. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31704]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys,Bioimaging Ctr, Shanghai 201800, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China 4.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China; 5.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China; 6.Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys,Div Phys Biol, Shanghai 201800, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, KZ,Wei, YH,Liu, WJ,et al. Mechanical Stress-Dependent Autophagy Component Release via Extracellular Nanovesicles in Tumor Cells[J]. ACS NANO,2019,13(4):4589—4602. |
| APA | Wang, KZ.,Wei, YH.,Liu, WJ.,Liu, L.,Guo, Z.,...&Li, B.(2019).Mechanical Stress-Dependent Autophagy Component Release via Extracellular Nanovesicles in Tumor Cells.ACS NANO,13(4),4589—4602. |
| MLA | Wang, KZ,et al."Mechanical Stress-Dependent Autophagy Component Release via Extracellular Nanovesicles in Tumor Cells".ACS NANO 13.4(2019):4589—4602. |
入库方式： OAI收割
来源：上海应用物理研究所
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