Programmable and printable Bacillus subtilis biofilms as engineered living materials
文献类型：期刊论文
| 作者 | Huang, JF; Liu, SY; Zhang, C; Wang, XY; Pu, JH; Ba, F; Xue, S; Ye, HF; Zhao, TX; Li, K |
| 刊名 | NATURE CHEMICAL BIOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 15期号:1页码:34—+ |
| 关键词 | PROTEIN STRATEGIES INSIGHTS STRAIN MATRIX FIBER |
| ISSN号 | 1552-4450 |
| DOI | 10.1038/s41589-018-0169-2 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Bacterial biofilms can be programmed to produce living materials with self-healing and evolvable functionalities. However, the wider use of artificial biofilms has been hindered by limitations on processability and functional protein secretion capacity. We describe a highly flexible and tunable living functional materials platform based on the TasA amyloid machinery of the bacterium Bacillus subtilis. We demonstrate that genetically programmable TasA fusion proteins harboring diverse functional proteins or domains can be secreted and can assemble into diverse extracellular nano-architectures with tunable physicochemical properties. Our engineered biofilms have the viscoelastic behaviors of hydrogels and can be precisely fabricated into microstructures having a diversity of three-dimensional (3D) shapes using 3D printing and microencapsulation techniques. Notably, these long-lasting and environmentally responsive fabricated living materials remain alive, self-regenerative, and functional. This new tunable platform offers previously unattainable properties for a variety of living functional materials having potential applications in biomaterials, biotechnology, and biomedicine. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31775]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai, Peoples R China; 2.ShanghaiTech Univ, Mat & Phys Biol Div, Sch Phys Sci & Technol, Shanghai, Peoples R China; 3.Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai, Peoples R China; 4.Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys,CAS Key Lab Interfacial P, Shanghai, Peoples R China; 5.MIT, Synthet Biol Grp, Res Lab Elect, 77 Massachusetts Ave, Cambridge, MA 02139 USA 6.Shanghai Jiao Tong Univ, Inst Mol Med, Sch Med, Renji Hosp, Shanghai, Peoples R China; 7.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Sch Med, Renji Hosp, Shanghai, Peoples R China; 8.EastChina Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai, Peoples R China; 9.East China Normal Univ, Sch Life Sci, Shanghai, Peoples R China; 10.East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Huang, JF,Liu, SY,Zhang, C,et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials[J]. NATURE CHEMICAL BIOLOGY,2019,15(1):34—+. |
| APA | Huang, JF.,Liu, SY.,Zhang, C.,Wang, XY.,Pu, JH.,...&Zhong, C.(2019).Programmable and printable Bacillus subtilis biofilms as engineered living materials.NATURE CHEMICAL BIOLOGY,15(1),34—+. |
| MLA | Huang, JF,et al."Programmable and printable Bacillus subtilis biofilms as engineered living materials".NATURE CHEMICAL BIOLOGY 15.1(2019):34—+. |
入库方式： OAI收割
来源：上海应用物理研究所
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