Redox Engineering of Cytochrome c using DNA Nanostructure-Based Charged Encapsulation and Spatial Control
文献类型：期刊论文
| 作者 | Ge, ZL; Su, ZM; Simmons, CR; Li, J; Jiang, SX; Li, W; Yang, Y; Liu, Y; Chiu, W; Fan, CH |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2019 |
| 卷号 | 11期号:15页码:13874—13880 |
| 关键词 | ELECTRON-TRANSFER ORIGAMI NANOSTRUCTURES PROTEIN FILMS GOLD IMMOBILIZATION ORGANIZATION VOLTAMMETRY MONOLAYERS COMPLEXES DELIVERY |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.8b07101 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Three-dimensional (3D) DNA nanostructures facilitate the directed self-assembly of various objects with designed patterns with nanometer scale addressability. Here, we report the enhancement of cytochrome c (cyt c) redox activity by using a designed 3D DNA nanostructure attached to a gold electrode to spatially control the position of cyt c within the tetrahedral framework. Charged encapsulation and spatial control result in the significantly increased redox potential and enhanced electron transfer of this redox protein when compared to cyt c directly adsorbed on the gold surface. Two different protein attachment sites on one double stranded edge of a DNA tetrahedron were used to position cyt c inside and outside of the cage. Cyt c at both binding sites show similar redox potential shift and only slight difference in the electron transfer rate, both orders of magnitude faster than the cases when the protein was directly deposited on the gold electrode, likely due to an effective electron transfer pathway provided by the stabilization effect of the protein created by the DNA framework. This study shows great potential of using structural DNA nanotechnology for spatial control of protein positioning on electrode, which opens new routes to engineer redox proteins and interface microelectronic devices with biological function. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31795]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Arizona State Univ, Dept Chem & Biochem, Biodesign Inst, Ctr Mol Design & Biomimet, Tempe, AZ 85287 USA; 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, Shanghai 201800, Peoples R China; 4.Stanford Univ, Natl Ctr Macromol Imaging, Dept Bioengn, Stanford, CA 94305 USA; 5.Stanford Univ, James H Clark Ctr, Dept Microbiol & Immunol, Stanford, CA 94305 USA |
| 推荐引用方式 GB/T 7714 | Ge, ZL,Su, ZM,Simmons, CR,et al. Redox Engineering of Cytochrome c using DNA Nanostructure-Based Charged Encapsulation and Spatial Control[J]. ACS APPLIED MATERIALS & INTERFACES,2019,11(15):13874—13880. |
| APA | Ge, ZL.,Su, ZM.,Simmons, CR.,Li, J.,Jiang, SX.,...&Yan, H.(2019).Redox Engineering of Cytochrome c using DNA Nanostructure-Based Charged Encapsulation and Spatial Control.ACS APPLIED MATERIALS & INTERFACES,11(15),13874—13880. |
| MLA | Ge, ZL,et al."Redox Engineering of Cytochrome c using DNA Nanostructure-Based Charged Encapsulation and Spatial Control".ACS APPLIED MATERIALS & INTERFACES 11.15(2019):13874—13880. |
入库方式： OAI收割
来源：上海应用物理研究所
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