Single-molecule measurement and bioinformatics analysis suggest a preferred orientation of human coagulation factor VIII on hydrophobic interfaces
文献类型：期刊论文
| 作者 | Cheng, J; Geng, F; Hu, J; Lu, JH |
| 刊名 | BIOPHYSICAL CHEMISTRY
 |
| 出版日期 | 2019 |
| 卷号 | 248期号:-页码:9—15 |
| 关键词 | PROTEIN ADSORPTION DOMAIN ACTIVATION SURFACES |
| ISSN号 | 0301-4622 |
| DOI | 10.1016/j.bpc.2019.03.001 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Investigating the adsorption behavior of coagulation proteins on interfaces will contribute to better understating blood clotting and to the development of biocompatible materials. In this work, atomic force microscopy (AFM)-based peakforce quantitative nanomechanical mapping (PF-QNM) was combined with bioinformatics tool to study the adsorption and orientation of coagulation factor VIII (FVIII) on both hydrophilic and hydrophobic interfaces by the height and mechanical measurement of single protein molecules. We found that interfacial hydrophilicity/hydrophobicity greatly influence the heights and Young's modulus of individual proteins. Compared to on the hydrophilic mica surface, FVIII proteins appear bigger vertical sizes while similar lateral sizes on the HOPG surface. The water accessible surface area analysis indicate stronger apolar properties C1 and C2 domains than others, suggesting a preferred orientation through the strong hydrophobic interactions between HOPG and the hydrophobic residues interface of the protein domains. These results provide novel insights on the adsorption and binding mechanism of the FVIII on cell membrane and will be helpful for the design of anticoagulant materials. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31817]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangiang Lab, Shanghai 201210, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China; 3.Binzhou Med Univ, Sch Pharm, Yantai 300064, Peoples R China; 4.Univ Chinese Acad Sci Beijing, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, J,Geng, F,Hu, J,et al. Single-molecule measurement and bioinformatics analysis suggest a preferred orientation of human coagulation factor VIII on hydrophobic interfaces[J]. BIOPHYSICAL CHEMISTRY,2019,248(-):9—15. |
| APA | Cheng, J,Geng, F,Hu, J,&Lu, JH.(2019).Single-molecule measurement and bioinformatics analysis suggest a preferred orientation of human coagulation factor VIII on hydrophobic interfaces.BIOPHYSICAL CHEMISTRY,248(-),9—15. |
| MLA | Cheng, J,et al."Single-molecule measurement and bioinformatics analysis suggest a preferred orientation of human coagulation factor VIII on hydrophobic interfaces".BIOPHYSICAL CHEMISTRY 248.-(2019):9—15. |
入库方式： OAI收割
来源：上海应用物理研究所
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