Stepping gating of ion channels on nanoelectrode via DNA hybridization for label-free DNA detection
文献类型：期刊论文
| 作者 | Zhao, HP; Ye, DK; Mao, XH; Li, F; Xu, JQ; Li, M; Zuo, XL |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2019 |
| 卷号 | 133期号:-页码:141—146 |
| 关键词 | GOLD NANOPARTICLES DOPAMINE RELEASE SMALL MOLECULES PROTEINS CURRENTS |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2019.03.038 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Natural ion channels on cell membrane can gate the transport of ions and molecules by the conformational alteration of transmembrane proteins to regulate the normal physiological activities of cells. Inspired by the similarity of the conformation change under specific stimuli, here we introduce an ion channel gating model on a single nanoelectrode by anchoring DNA-gated switches on the very nanotip of gold nanoelectrode to mimic the response-to-stimulus behaviors of ion channels on bio-membranes. The surface-tethered DNA ion channels can be switched on by the Watson-Crick base pairing, which can alter the conformation of the tethered DNA from lying state to upright state. And these conformational alterations of the anchored DNA switches can effectively gate the transport of potassium ferricyanide onto the electrode interface. By continuously initiating the gates with DNA of different concentrations, we achieved the stepping gating of ion channels on a single nanoelectrode. Further, we demonstrated that the ion gating system on nanoelectrode showed excellent sensing performance. For example, the response kinetic was very fast with the signal saturation time of similar to 1 min, the reproducibility of the OFF/ON switch was robust enough to sustain for two cycles, and simultaneously, the specificity was high enough to distinguish complementary DNA and non-complementary DNA. When used for label-free DNA detection, the limit of detection can be as low as 10 pM. This study provides a promising avenue to achieve label free and real-time detection of multiple biomolecules. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31827]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Univ, Coll Sci, Dept Chem, NEST Lab, Shanghai 200444, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai 200127, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai 201800, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, HP,Ye, DK,Mao, XH,et al. Stepping gating of ion channels on nanoelectrode via DNA hybridization for label-free DNA detection[J]. BIOSENSORS & BIOELECTRONICS,2019,133(-):141—146. |
| APA | Zhao, HP.,Ye, DK.,Mao, XH.,Li, F.,Xu, JQ.,...&Zuo, XL.(2019).Stepping gating of ion channels on nanoelectrode via DNA hybridization for label-free DNA detection.BIOSENSORS & BIOELECTRONICS,133(-),141—146. |
| MLA | Zhao, HP,et al."Stepping gating of ion channels on nanoelectrode via DNA hybridization for label-free DNA detection".BIOSENSORS & BIOELECTRONICS 133.-(2019):141—146. |
入库方式： OAI收割
来源：上海应用物理研究所
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