Submicrometre Superstructure Co-assembled from Protein and DNA
文献类型：期刊论文
| 作者 | Dai, JB; Zhang, LX; Mao, XH; Zhao, Y; Li, K; Gu, PL; Guo, LJ; Li, J; Zhong, C; Fan, CH |
| 刊名 | ACTA POLYMERICA SINICA
 |
| 出版日期 | 2019 |
| 卷号 | 50期号:4页码:359—365 |
| 关键词 | ARRAYS VIRUS RNA |
| ISSN号 | 1000-3304 |
| DOI | 10.11777/j.issn1000-3304.2018.18251 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Functional supramolecular complexes co-assembled with multiple proteins and nucleic acids are ubiquitous in nature, such as ribosome and viruses. It is fundamentally important to understand and utilize these heterogeneous structures. Here, we reported a protein-DNA submicrometre superstructure fabricated from the self-assembly of protein CsgA and DNA nanostructures. By inspecting the physiological conditions of protein-DNA co-assembly, we found that the originally soluble CsgA could polymerize into insoluble fibers in a particular buffer (30 mmol/L Tris-HCl, 450 mmol/L NaCl, pH = 7.2), and such fibers benefited the storage of tetrahedron DNA nanostructure (TDN). Concentration and fibrillation time were optimized for the aggregation-free conversion of CsgA into amyloid fibers. Specifically, the optimum conversion of monomeric CsgA into micrometer-scale fibers could be realized at a concentration of 5 mu mol/L. Meanwhile, atomic force microscopy (AFM) suggested that CsgA assembled into mature fibers in 5 days and formed larger aggregates after 7 days. The height of mature fibers and aggregates was about 3.9 and 6.1 nm, respectively. Afterwards, TDN was modified with NTA molecule and conjugated to CsgA through the chelation of Ni2+, His-tag, and NTA. A submicrometre complex CsgA fiber-dTDN was further generated by hybridizing two copies of TDN in dimeric structure (dTDN) through beta-sheet interactions and Watson-Crick hybridization. This approach could fabricate a series of dTDN structures precisely without inducing the random aggregation of molecules. The yield of up to 44% was higher than that obtained from the direct connection of DNA modules via DNA technology. In summary, our findings demonstrated that CsgA fibers could act as a sort of novel scaffold for the assembly of protein-templated DNA nanostructure. Particularly, this model provides a deep insight into the generation of functional superstructures through self-assembly of protein and DNA-based building blocks. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31837]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Jiading Dist Cent Hosp, Shanghai 201800, Peoples R China; 4.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, JB,Zhang, LX,Mao, XH,et al. Submicrometre Superstructure Co-assembled from Protein and DNA[J]. ACTA POLYMERICA SINICA,2019,50(4):359—365. |
| APA | Dai, JB.,Zhang, LX.,Mao, XH.,Zhao, Y.,Li, K.,...&Wang, LH.(2019).Submicrometre Superstructure Co-assembled from Protein and DNA.ACTA POLYMERICA SINICA,50(4),359—365. |
| MLA | Dai, JB,et al."Submicrometre Superstructure Co-assembled from Protein and DNA".ACTA POLYMERICA SINICA 50.4(2019):359—365. |
入库方式： OAI收割
来源：上海应用物理研究所
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