Substrate Metabolism-Driven Assembly of High-Quality CdSxSe1-x Quantum Dots in Escherichia coli: Molecular Mechanisms and Bioimaging Application
文献类型：期刊论文
| 作者 | Tian, LJ; Min, Y; Li, WW; Chen, JJ; Zhou, NQ; Zhu, TT; Li, DB; Ma, JY; An, PF; Zheng, LR |
| 刊名 | ACS NANO
 |
| 出版日期 | 2019 |
| 卷号 | 13期号:5页码:5841—5851 |
| 关键词 | DYNAMICS SIMULATIONS OXIDATIVE STRESS NANOPARTICLES GLUTATHIONE BACTERIA BIOSYNTHESIS GLUTAREDOXINS INTEGRATION CADMIUM SYSTEM |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.9b01581 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Biosynthesis offers opportunities for cost-effective and sustainable production of semiconductor quantum dots (QDs), but is currently restricted by poor controllability on the synthesis process, resulting from limited knowledge on the assembly mechanisms and the lack of effective control strategies. In this work, we provide molecular-level insights into the formation mechanism of biogenic QDs (Bio-QDs) and its connection with the cellular substrate metabolism in Escherichia coli. Strengthening the substrate metabolism for producing more reducing power was found to stimulate the production of several reduced thiol-containing proteins (including glutaredoxin and thioredoxin) that play key roles in Bio-QDs assembly. This effectively diverted the transformation route of the selenium (Se) and cadmium (Cd) metabolic from Cd-3(PO4)(2) formation to CdSxSe1-x QDs assembly, yielding fine-sized (2.0 +/- 0.4 nm), high-quality Bio-QDs with quantum yield (5.2%) and fluorescence lifetime (99.19 ns) far exceeding the existing counterparts. The underlying mechanisms of Bio-QDs crystallization and development were elucidated by density functional theory calculations and molecular dynamics simulation. The resulting Bio-QDs were successfully used for bioimaging of cancer cells and tumor tissue of mice without extra modification. Our work provides fundamental knowledge on the Bio-QDs assembly mechanisms and proposes an effective, facile regulation strategy, which may inspire advances in controlled synthesis and practical applications of Bio-QDs as well as other bionanomaterials. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31838]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Univ Sci & Technol China, Dept Appl Chem, CAS Key Lab Urban Pollutant Convers, Hefei 230026, Anhui, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China; 3.Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Lab, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, LJ,Min, Y,Li, WW,et al. Substrate Metabolism-Driven Assembly of High-Quality CdSxSe1-x Quantum Dots in Escherichia coli: Molecular Mechanisms and Bioimaging Application[J]. ACS NANO,2019,13(5):5841—5851. |
| APA | Tian, LJ.,Min, Y.,Li, WW.,Chen, JJ.,Zhou, NQ.,...&Yu, HQ.(2019).Substrate Metabolism-Driven Assembly of High-Quality CdSxSe1-x Quantum Dots in Escherichia coli: Molecular Mechanisms and Bioimaging Application.ACS NANO,13(5),5841—5851. |
| MLA | Tian, LJ,et al."Substrate Metabolism-Driven Assembly of High-Quality CdSxSe1-x Quantum Dots in Escherichia coli: Molecular Mechanisms and Bioimaging Application".ACS NANO 13.5(2019):5841—5851. |
入库方式： OAI收割
来源：上海应用物理研究所
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