Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina
文献类型：期刊论文
| 作者 | Yan, Wenjie5; Liu, Jiao4; Seng, Samphal3; Zhou, Bin5; Ding, Kuke1,2 |
| 刊名 | ACTA OCEANOLOGICA SINICA
 |
| 出版日期 | 2019-10-01 |
| 卷号 | 38期号:10页码:111-115 |
| 关键词 | Zostera marina antioxidant enzyme glutathione S-transferase (GST) temperature enzyme activity |
| ISSN号 | 0253-505X |
| DOI | 10.1007/s13131-019-1429-z |
| 通讯作者 | Ding, Kuke(shouding@ccmu.edu.cn) |
| 英文摘要 | The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn. |
| 资助项目 | Open Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology, State Oceanic Administration[201704] ; Shandong Provincial Natural Science Foundation of China[ZR2018MD020] |
| WOS研究方向 | Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000490598500013 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/163226]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Ding, Kuke |
| 作者单位 | 1.Chinese Ctr Dis Control & Prevent, Key Lab Radiol Protect & Nucl Emergency, Beijing 100088, Peoples R China 2.Chinese Ctr Dis Control & Prevent, Natl Inst Radiol Protect, Beijing 100088, Peoples R China 3.Royal Univ Agr, Fac Fisheries & Aquaculture, Phnom Penh 12101, Cambodia 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 5.Ocean Univ China, Coll Marine Life Sci, Dept Marine Ecol, Qingdao 266003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Wenjie,Liu, Jiao,Seng, Samphal,et al. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. ACTA OCEANOLOGICA SINICA,2019,38(10):111-115. |
| APA | Yan, Wenjie,Liu, Jiao,Seng, Samphal,Zhou, Bin,&Ding, Kuke.(2019).Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina.ACTA OCEANOLOGICA SINICA,38(10),111-115. |
| MLA | Yan, Wenjie,et al."Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina".ACTA OCEANOLOGICA SINICA 38.10(2019):111-115. |
入库方式： OAI收割
来源：海洋研究所
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