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Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity

文献类型：期刊论文

作者Sun Yuhao1,2; Chen Xiaolin2; Liu Song2; Yu Huahua2; Li Rongfeng2; Wang Xueqin2; Qin Yukun2; Li Pengcheng2
刊名JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2018-05-01
卷号36期号:3页码:882-891
关键词anticoagulant activity Sargassum fusiforme low molecular weight degradation
ISSN号2096-5508
DOI10.1007/s00343-018-7089-6
通讯作者Chen Xiaolin(chenxl@qdio.ac.cn) ; Li Pengcheng(pcli@qdio.ac.cn)
英文摘要Heparin has been used as an anticoagulant drug for many years, but it has significant side effects. In the search far good substitutes, low molecular weight (MW) polysaccharides from Sargassum fusiforme have been examined and confirmed to possess biological activities. Here, S. fusiforme polysaccharides (SFP) were extracted and subjected to a hydrogen peroxide (H2O2) oxidation method for the preparation of low-MW SFP (LSFP). The effects of temperature, pH, and H2O2 concentration on the degradation process were also examined. Several LSFP of 36, 9, 5.7, and 2.7 kDa were obtained under different conditions, and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin (APTT), prothrombin (PT) and thrombin times (TT) significantly, indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic, extrinsic, and common coagulation pathways. As these effects were related to the MW of the polysaccharides in APTT and TT but not in PT, the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined effects. The details of this mechanism require further verification.
资助项目Ocean Public Welfare Scientific Research Project, State Oceanic Administration of the People's Republic of China[201505033] ; Qingdao People's Livelihood Science and Technology Program[16-6-2-41-nsh]
WOS研究方向Marine & Freshwater Biology ; Oceanography
语种英语
WOS记录号WOS:000438791100026
出版者SCIENCE PRESS
源URL[http://ir.qdio.ac.cn/handle/337002/163507]  
专题中国科学院海洋研究所
通讯作者Chen Xiaolin; Li Pengcheng
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Sun Yuhao,Chen Xiaolin,Liu Song,et al. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(3):882-891.
APA Sun Yuhao.,Chen Xiaolin.,Liu Song.,Yu Huahua.,Li Rongfeng.,...&Li Pengcheng.(2018).Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(3),882-891.
MLA Sun Yuhao,et al."Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.3(2018):882-891.

入库方式： OAI收割

来源：海洋研究所

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