Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity
文献类型:期刊论文
作者 | Tan, Xungang2,3; Xu, Peng1,2,3; Zhang, Yuqing1,2,3; Zhang, Pei-Jun2,3 |
刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY |
出版日期 | 2019-10-01 |
卷号 | 236页码:9 |
ISSN号 | 1096-4959 |
关键词 | Olive flounder Myogenesis Somites mrf4 promoter Zebrafish |
DOI | 10.1016/j.cbpb.2019.110310 |
通讯作者 | Tan, Xungang(tanx@qdio.ac.cn) |
英文摘要 | Myogenic regulatory factor 4 (MRF4) is a basic helix-loop-helix (bHLH) transcription factor that plays crucial roles in myoblast differentiation and maturation. Here, we report the isolation of the olive flounder (Paralichthys olivaceus) mrf4 gene and the spatiotemporal analysis of its expression patterns. Sequence analysis indicated that flounder mrf4 shared a similar structure with other vertebrate MRF4, including the conserved bHLH domain. Flounder mrf4 contains 3 exons and 2 introns. Sequence alignment and phylogenetic analysis showed that it was highly homologous with Salmo salar, Danio rerio, Takifugu rubripes, and Tetraodon nigroviridis mrf4. Flounder mrf4 was first expressed in the medial region of somites that give rise to slow muscles, and later spread to the lateral region of somites that give rise to fast muscles. Mrf4 transcript levels decreased significantly in mature somites in the trunk region, and expression could only be detected in the caudal somites, consistent with the timing of somite maturation. Transient expression analysis showed that the 506 bp flounder mrf4 promoter was sufficient to direct muscle-specific GFP expression in zebrafish embryos. |
资助项目 | National Sciences of Foundation of China[31672636] ; National Sciences of Foundation of China[31128017] |
WOS研究方向 | Biochemistry & Molecular Biology ; Zoology |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE INC |
WOS记录号 | WOS:000484655000003 |
源URL | [http://ir.qdio.ac.cn/handle/337002/162702] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
通讯作者 | Tan, Xungang |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Tan, Xungang,Xu, Peng,Zhang, Yuqing,et al. Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2019,236:9. |
APA | Tan, Xungang,Xu, Peng,Zhang, Yuqing,&Zhang, Pei-Jun.(2019).Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,236,9. |
MLA | Tan, Xungang,et al."Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 236(2019):9. |
入库方式: OAI收割
来源:海洋研究所
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