Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms
文献类型：期刊论文
| 作者 | Chen, Zhihang1,2,3; Wang, Xiuliang2,3; Li, Shuang1,2,3 ; Yao, Jianting2,3; Shao, Zhanru2,3; Duan, Delin2,3
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2019-08-02 |
| 卷号 | 20期号:16页码:14 |
| 关键词 | Tic20 EF-hand Phaeodactylum tricornutum Saccharina japonica subcellular localization transmembrane topology |
| ISSN号 | 1661-6596 |
| DOI | 10.3390/ijms20164000 |
| 通讯作者 | Wang, Xiuliang(xlwang@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn) |
| 英文摘要 | Tic20 is an important translocon protein that plays a role in protein transport in the chloroplast. The sequence of Tic20 was determined in the lower brown alga Saccharina japonica. Structural analysis of SjTic20 revealed a noncanonical structure consisting of an N-terminal non-cyanobacterium-originated EF-hand domain (a helix-loop-helix structural domain) and a C-terminal cyanobacterium-originated Tic20 domain. Subcellular localization and transmembrane analysis indicated that SjTic20 featured an M-type N-in-C-in-terminal orientation, with four transmembrane domains in the innermost membrane of the chloroplast in the microalga Phaeodactylum tricornutum, and the EF-hand domain was entirely extruded into the chloroplast stroma. Our study provides information on the structure, localization, and topological features of SjTic20, and further functional analysis of SjTic20 in S. japonica is needed. |
| 资助项目 | National Nature Science Foundation of China[31772848] ; National Nature Science Foundation of China[31272660] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; [2017YFE0130900] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000484411100157 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/162768]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Xiuliang; Duan, Delin |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhihang,Wang, Xiuliang,Li, Shuang,et al. Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(16):14. |
| APA | Chen, Zhihang,Wang, Xiuliang,Li, Shuang,Yao, Jianting,Shao, Zhanru,&Duan, Delin.(2019).Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(16),14. |
| MLA | Chen, Zhihang,et al."Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.16(2019):14. |
入库方式： OAI收割
来源：海洋研究所
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