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The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila

文献类型：期刊论文

作者Wang, Jingjing5,6; Yang, Bin4,5; Wang, Weilin3; Song, Xiaorui3; Jiang, Qiufen5; Qiu, Limei5; Wang, Lingling2,3,4; Song, Linsheng1,3,4
刊名FRONTIERS IN IMMUNOLOGY
出版日期2019-08-28
卷号10页码:12
关键词immune priming Eriocheir sinensis Aeromonas hydrophila phagocytosis immune protection
ISSN号1664-3224
DOI10.3389/fimmu.2019.02041
通讯作者Song, Linsheng(lshsong@dlou.edu.cn)
英文摘要Accumulating evidences suggest that the enhanced immune responses and increased protection against bacteria-induced mortality can be initiated after the primary exposure to various microbial communities and their components in various organisms including commercially valuable crustaceans. In the present study, the survival rate and immune responses of Chinese mitten crab Eriocheir sinensis were determined after an immune priming (IP) with formalin-killed Aeromonas hydrophila and an immune challenge (ICH) with the same but live pathogen (Ah group). A group in which the animals received a salt injection prior to challenge was maintained as control (Ns group). In the present study, it was shown that an IP with killed A. hydrophila can significantly protect the crabs against the ICH with a lethal dose of the live pathogen. The increased survival was associated with elevated rate and duration of phagocytosis. The antibacterial activity of the serum was significantly increased in Ah group compared to that in Ns group. Significant changes of phenoloxidase (PO) activities were also found between Ah and Ns group but not in Ah group between IP and ICH. No significant changes of lysozyme were found in Ah and NS group during the whole experiment except 3 h after IP. In addition, the levels of transcripts and protein of Dscam were increased in hemocytes of the crabs from Ah group. All the results suggested that a primary immune priming with a particular killed pathogen could induce an enhanced immunity in crabs when they were encountered secondly with the same live pathogen. The evidences of elevated immune protections in crabs would contribute to better understand the mechanism of immune priming in invertebrates.
资助项目National Key RD Program[2018YFD0900606] ; National Natural Science Foundation of China[31530069] ; National Natural Science Foundation of China[31502202] ; AoShan Talents Cultivation Program by Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Shandong Province Natural Science Foundation[ZR2014CL035] ; Advanced Talents Foundation of Qingdao Agricultural University[6631114317] ; First class fishery discipline programme in Shandong Province, China ; Dalian High Level Talent Innovation Support Program[2015R020]
WOS研究方向Immunology
语种英语
WOS记录号WOS:000482915500002
出版者FRONTIERS MEDIA SA
源URL[http://ir.qdio.ac.cn/handle/337002/162843]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Song, Linsheng
作者单位1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China
2.Dalian Ocean Univ, Dalian Key Lab Aquat Anim Dis Prevent & Control, Dalian, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
6.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Jingjing,Yang, Bin,Wang, Weilin,et al. The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila[J]. FRONTIERS IN IMMUNOLOGY,2019,10:12.
APA Wang, Jingjing.,Yang, Bin.,Wang, Weilin.,Song, Xiaorui.,Jiang, Qiufen.,...&Song, Linsheng.(2019).The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila.FRONTIERS IN IMMUNOLOGY,10,12.
MLA Wang, Jingjing,et al."The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila".FRONTIERS IN IMMUNOLOGY 10(2019):12.

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来源：海洋研究所

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