Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis
文献类型：期刊论文
| 作者 | Du, Jing1,4; Liu, Yuan4; Song, Chengwen1,4 ; Cui, Zhaoxia2,3
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| 刊名 | GENE
 |
| 出版日期 | 2019-08-20 |
| 卷号 | 710页码:1-8 |
| 关键词 | Transcriptome Sex determination Embryonic development Eriocheir sinensis |
| ISSN号 | 0378-1119 |
| DOI | 10.1016/j.gene.2019.05.021 |
| 通讯作者 | Cui, Zhaoxia(cuizhaoxia@nbu.edu.cn) |
| 英文摘要 | Investigation of sex determination system in Eriocheir sinensis is important because of sex-dimorphism in its growth traits. However, little information about the sex-related genes in embryonic development stages were exposed. To obtain more information of sex determination in Chinese mitten crab, we performed the transcriptome analysis in embryonic development stage (fertilized egg stage, cleavage stage, blastula stage, gastrula stage and heartbeat stage) of Chinese mitten crab using nextgeneration sequencing technology. Thirty-one of 32,088 annotated unigenes were identified as sex-related genes including 16 genes involved in primary sex determination in model organisms and 8 genes of SOX family and 7 genes of DMRT gene family. Heatmap based on the RPKM value in five embryonic development stages indicated that these genes were clustered into two branches. Analysis of the differentially expressed 12 genes, including 3 genes of SOX family, 3 genes of DMRT gene family and 6 genes involved in primary sex determination in model organisms, showed significantly difference between the first three periods (fertilized egg stage-cleavage stage-blastula stage) and the last two periods (gastrula stage-heartbeat stage) and all 12 genes were up-regulated after blastula stage. In conclusion, we inferred that sex determination might be initiated after blastula stage in E. sinensis. Transcriptome analysis from embryonic development stage could provide a background information for further investigation in sex determination of Eriocheir sinensis. |
| 资助项目 | National Key Research and Development Program of China[2018YFD0900303] ; Ten Thousand Talents Program |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000483423500001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/162864]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Cui, Zhaoxia |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Ningbo Univ, Ningbo 315020, Zhejiang, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Jing,Liu, Yuan,Song, Chengwen,et al. Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis[J]. GENE,2019,710:1-8. |
| APA | Du, Jing,Liu, Yuan,Song, Chengwen,&Cui, Zhaoxia.(2019).Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis.GENE,710,1-8. |
| MLA | Du, Jing,et al."Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis".GENE 710(2019):1-8. |
入库方式： OAI收割
来源：海洋研究所
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