Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus
文献类型：期刊论文
| 作者 | Wu, Zhihao3,4; Wang, Lijuan3,4 ; Lu, Yunliang2; Zhu, Xiangping2; Yue, Xinlu1; You, Feng3,4
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| 刊名 | FRONTIERS IN MARINE SCIENCE
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| 出版日期 | 2019-10-16 |
| 卷号 | 6页码:12 |
| 关键词 | turbot Scophthalmus maximus tetraploid artificial induction first cleavage index genetic diversity |
| DOI | 10.3389/fmars.2019.00637 |
| 通讯作者 | You, Feng(youfeng@qdio.ac.cn) |
| 英文摘要 | Artificial tetraploid induction is one of the important techniques of fish chromosome manipulation, and it is the first step for triploid breeding. There are a few reports to artificial induction of tetraploid in marine fish. The induction and survival rates were usually low. We firstly optimized the tetraploid induction conditions in turbot Scophthalmus maximus, one of the most important maricultural fish in China and Europe. For the initiate time of treatment, which is the most important factor in tetraploid induction, the first cleavage index (FCI) was used to reduce the influences of genetic origin and environment factors. Overall, the optimal initiation time for pressure shock was 15 min before the first cleavage at 14.8-15.5 degrees C. The optimal treatment pressure and treatment duration were 67.5 MPa and 6 min. The regression equation prediction model was: The optimal initiation time = 0.982 FCI - 12.182 or the optimal initiation time = 0.85 FCI. Then two tetraploid induction (4n1 and 4n2) populations were obtained under the optimal conditions with diploid controls (2n1 and 2n2). The induction rates in tetraploid induction (4n) populations at hatched larvae stage could reach 100%. The genetic structure of these two 4n populations was also studied. Two to four alleles in each locus were detected in diploid (2n) and 4n populations, respectively. Private alleles were only appeared at locus Sma-USC21, with two alleles lost in 4n populations. Eleven and fourteen loci in 2n and 4n populations respectively showed a negative genetic deviation index. 3D-FCA analysis showed that the two 2n and two 4n populations have obvious differences. The numbers of locus deviating from Hardy-Weinberg equilibrium in 2n1, 4n1, 2n2, and 4n2 populations were 6, 9, 12, and 7, respectively. Overall, 12 loci in either 2n or 4n population deviated from Hardy-Weinberg equilibrium. Tetraploid induction population showed lower heterozygosity and higher heterozygote deletion. |
| 资助项目 | National Natural Science Foundation of China[31502156] ; National Natural Science Foundation of China[41276171] ; Key Research and Development Plan of Shandong Province[2017CXGC0101] ; National Key R&D Program of China[2018YFD0901202] |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000490444800001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/163253]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | You, Feng |
| 作者单位 | 1.Shenghang Aquat Sci & Technol Co Ltd, Weihai, Peoples R China 2.Qingdao Agr Univ Qingdao, Marine Sci & Engineer Coll, Qingdao, Shandong, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Zhihao,Wang, Lijuan,Lu, Yunliang,et al. Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus[J]. FRONTIERS IN MARINE SCIENCE,2019,6:12. |
| APA | Wu, Zhihao,Wang, Lijuan,Lu, Yunliang,Zhu, Xiangping,Yue, Xinlu,&You, Feng.(2019).Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus.FRONTIERS IN MARINE SCIENCE,6,12. |
| MLA | Wu, Zhihao,et al."Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus".FRONTIERS IN MARINE SCIENCE 6(2019):12. |
入库方式： OAI收割
来源：海洋研究所
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