Effects of a Sudden Drop in Salinity on Immune Response Mechanisms of Anadara kagoshimensis
文献类型：期刊论文
| 作者 | Zhang, Mo4; Li, Li3; Liu, Ying2,4 ; Gao, Xiaolong1
|
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2019-09-02 |
| 卷号 | 20期号:18页码:21 |
| 关键词 | Anadara kagoshimensis salinity transcriptomics cellular immunity real-time quantitative PCR |
| DOI | 10.3390/ijms20184365 |
| 通讯作者 | Gao, Xiaolong(xlgao@xmu.edu.cn) |
| 英文摘要 | In this experiment, the effects of a sudden drop of salinity on the immune response mechanisms of the ark shell Anadara kagoshimensis were examined by simulating the sudden drop of salinity that occurs in seawater after a rainstorm. Additionally, the differentially expressed genes (DEGs) were identified using transcriptome sequencing. When the salinity dropped from 30 parts per thousand (S30) to 14 parts per thousand (S14), the phagocytic activity of blood lymphocytes, the O-2(-) levels produced from respiratory burst, the content of reactive oxygen species, and the activities of lysozymes and acid phosphatases increased significantly, whereas the total count of blood lymphocytes did not increase. Total count of blood lymphocytes in 22 parts per thousand salinity (S22) was significantly higher than that in any other group. The raw data obtained from sequencing were processed with Trimmomatic (Version 0.36). The expression levels of unigenes were calculated using transcripts per million (TPM) based on the effects of sequencing depth, gene length, and sample on reads. Differential expression analysis was performed using DESeq (Version 1.12.4). Transcriptome sequencing revealed 269 (101 up-regulated, 168 down-regulated), 326 (246 up-regulated, 80 down-regulated), and 185 (132 up-regulated, 53 down-regulated) significant DEGs from comparison of the S14 vs. S22, S22 vs. S30, and S14 vs. S30 groups, respectively. Gene Ontology enrichment analysis of the DEGs in these salinity comparison groups revealed that the cellular amino acid metabolic process, the regulation of protein processing, the regulation of response to stress, and other terms were significantly enriched. Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that nucleotide-binding, oligomerization domain (NOD)-like receptor signaling pathway (ko04621), apoptosis-multiple species (ko04215), Toll and Imd signaling pathway (ko04624), NF-kappa B signaling pathway (ko04064), apoptosis (ko04210), and focal adhesion (ko04510) were significantly enriched in all salinity comparison groups. qRT-PCR verification of 12 DEGs in the above six pathways was conducted, and the results were consistent with the transcriptome sequencing results in terms of up-regulation and down-regulation, which illustrates that the transcriptome sequencing data are credible. These results were used to preliminarily explore the effects of a sudden drop of salinity on blood physiological and biochemical indexes and immunoregulatory mechanisms of A.kagoshimensis. They also provide a theoretical basis for the selection of bottom areas optimal for release and proliferation of A.kagoshimensis required to restore the declining populations of this species. |
| 资助项目 | China Postdoctoral Science Foundation[2019M650153] ; Modern Agro-industry Technology Research System in Shandong Province[SDAIT-14-04] ; State Key Laboratory of Marine Environmental Science at Xiamen University |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000489100500044 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/163425]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Gao, Xiaolong |
| 作者单位 | 1.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Fujian, Peoples R China 2.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian 116023, Peoples R China 3.Marine Biol Inst Shandong Prov, Qingdao 266104, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Mo,Li, Li,Liu, Ying,et al. Effects of a Sudden Drop in Salinity on Immune Response Mechanisms of Anadara kagoshimensis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(18):21. |
| APA | Zhang, Mo,Li, Li,Liu, Ying,&Gao, Xiaolong.(2019).Effects of a Sudden Drop in Salinity on Immune Response Mechanisms of Anadara kagoshimensis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(18),21. |
| MLA | Zhang, Mo,et al."Effects of a Sudden Drop in Salinity on Immune Response Mechanisms of Anadara kagoshimensis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.18(2019):21. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。