中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei

文献类型:期刊论文

作者Yang, Feifei4; Li, Xuechun1,2,3,4; Li, Shihao2,3,4; Xiang, Jianhai2,3,4; Li, Fuhua2,3,4
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2020
卷号102页码:9
ISSN号0145-305X
关键词Litopenaeus vannamei WSSV Cuticle protein Chitin binding domain RNAi Interaction
DOI10.1016/j.dci.2019.103491
通讯作者Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
英文摘要As the most productive crustacean species in aquaculture, Litopenaeus vannamei is seriously threatened by white spot syndrome virus (WSSV), which has caused huge economic damage in the past decades. Shrimp cuticle proteins are the important components in the frontier target tissues, including cuticle and the chitinous lining of the digestive tract. In present study, a novel cuticle protein gene, named LvCPAP1, was isolated and demonstrated to play an important role in WSSV infection. The deduced amino acid sequence of LvCPAP1 contained a signal peptide and a conserved chitin-binding domain type 2 (ChBD2). Tissue distribution analysis revealed that LvCPAP1 was predominantly expressed in epidermis and stomach. The transcription levels of LvCPAP1 in epidermis and stomach were significantly regulated upon WSSV challenge. DsRNA silencing of LvCPAP1 decreased the in vivo WSSV copy numbers and the death rate of shrimp after WSSV infection, indicating that LvCPAP1 might facilitate WSSV invasion. In addition, the interaction between LvCPAP1 and the major envelop protein VP24 of WSSV was revealed by yeast two-hybrid system and further confirmed by dot blot and pull-down assays. The present study implied that cuticle protein LvCPAP1 might favor the entry process of WSSV, which provided new clues for understanding the role of cuticle proteins during virus infection.
资助项目China Agriculture Research System-48[CARS-48] ; China Postdoctoral Science Foundation[2017M612361] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017N004]
WOS研究方向Fisheries ; Immunology ; Zoology
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000491213900020
源URL[http://ir.qdio.ac.cn/handle/337002/163434]  
专题海洋研究所_实验海洋生物学重点实验室
中国科学院海洋大科学研究中心
通讯作者Li, Shihao; Li, Fuhua
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
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Yang, Feifei,Li, Xuechun,Li, Shihao,et al. A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,102:9.
APA Yang, Feifei,Li, Xuechun,Li, Shihao,Xiang, Jianhai,&Li, Fuhua.(2020).A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,102,9.
MLA Yang, Feifei,et al."A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 102(2020):9.

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来源:海洋研究所

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