An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria
文献类型：期刊论文
| 作者 | Chen, Hao2,3,5 ; Wang, Minxiao2,3,4,5; Zhang, Huan2,3,5; Wang, Hao2,3,5; Lv, Zhao1; Zhou, Li2,3,5; Zhong, Zhaoshan1,5; Lian, Chao2,3,5; Cao, Lei2,3,5; Li, Chaolun1,2,3,4,5
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2019-10-01 |
| 卷号 | 93页码:354-360 |
| 关键词 | Bathymodioline mussels Chemosymbiosis Pattern recognition receptors LRR protein Pull-down |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.07.032 |
| 通讯作者 | Li, Chaolun(lcl@qdio.ac.cn) |
| 英文摘要 | As domain species in seep and vent ecosystem, Bathymodioline mussels has been regarded as a model organism in investigating deep sea chemosymbiosis. However, mechanisms underlying their symbiosis with chemosynthetic bacteria, especially how the host recognizes symbionts, have remained largely unsolved. In the present study, a modified pull-down assay was conducted using enriched symbiotic methane-oxidation bacteria as bait and gill proteins of Bathymodiolus platifrons as a target to isolate pattern recognition receptors involved in the immune recognition of symbionts. As a result, a total of 47 proteins including BpLRR-1 were identified from the pull-down assay. It was found that complete cDNA sequence of BpLRR-1 contained an open reading frame of 1479 bp and could encode a protein of 492 amino acid residues with no signal peptide or transmembrane region but eight LRR motif and two EFh motif. The binding patterns of BpLRR-1 against microbial associated molecular patterns were subsequently investigated by surface plasmon resonance analysis and LPS pull-down assay. Consequently, BpLRR-1 was found with high binding affinity with LPS and suggested as a key molecule in recognizing symbionts. Besides, transcripts of BpLRR-1 were found decreased significantly during symbiont depletion assay yet increased rigorously during symbionts or nonsymbiotic Vibrio alginolyticus challenge, further demonstrating its participation in the chemosynthetic symbiosis. Collectively, these results suggest that BpLRR-1 could serve as an intracellular recognition receptor for the endosymbionts, providing new hints for understanding the immune recognition in symbiosis of B. platifrons. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; National Key Research and Development Program of China[2018YFC0310802] ; National Key Research and Development Program of China[2018YFC0310604] ; China Postdoctoral Science Foundation[2017LH023] ; Qingdao Postdoctoral Science Foundation |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000488998300041 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/163305]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Li, Chaolun |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 5.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hao,Wang, Minxiao,Zhang, Huan,et al. An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:354-360. |
| APA | Chen, Hao.,Wang, Minxiao.,Zhang, Huan.,Wang, Hao.,Lv, Zhao.,...&Li, Chaolun.(2019).An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria.FISH & SHELLFISH IMMUNOLOGY,93,354-360. |
| MLA | Chen, Hao,et al."An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria".FISH & SHELLFISH IMMUNOLOGY 93(2019):354-360. |
入库方式： OAI收割
来源：海洋研究所
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