COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)
文献类型：期刊论文
| 作者 | Lyu, Man2,4 ; Lei, Yanli2,4; Li, Tiegang1,2,3
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| 刊名 | JOURNAL OF FORAMINIFERAL RESEARCH
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 48期号:3页码:186-192 |
| ISSN号 | 0096-1191 |
| 通讯作者 | Lei, Yanli(leiyanli@qdio.ac.cn) ; Li, Tiegang() |
| 英文摘要 | We carried out a series of experiments to evaluate the efficiency of preserving DNA from porcelaneous foraminifera (Quinqueloculina spp.) and a second set to assess the effect of Rose Bengal staining on molecular processing. The first experimental setup assessed three methods of DNA preservation (air-drying, freezing with or without seawater, and Guanidine lysis buffer treatment with or without EDTA (Ethylenediaminetetraacetic acid)). Our study produced the following results: 1) there were no significant differences in DNA preservation when samples were air dried across a range of temperatures (20-120 degrees C); samples frozen at -20 degrees C appeared better preserved than at those frozen at -80 degrees C, and freezing without seawater appeared to produce better preservation than with seawater, though differences in freezing treatments were not significant (p>0.05); samples in Guanidine lysis buffer with EDTA and stored at -20 degrees C were well preserved (p<0.05); 2) sometimes, DNA was successfully extracted from samples stained with Rose Bengal. We recommend Guanidine lysis buffer with EDTA, stored at -20 degrees C for up to six weeks, as the best protocol for preservation of DNA from porcelaneous foraminifera. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11030104] ; National Science Foundation of China[41476043] ; National Science Foundation of China[41230959] ; National Program on 'Global Change and Air-Sea Interaction'[GASI-GEOGE-06-02] ; Continental Shelf Drilling Program of China[GZH201100202] ; Paul Bronnimann Foundation |
| WOS研究方向 | Paleontology |
| 语种 | 英语 |
| WOS记录号 | WOS:000439322600002 |
| 出版者 | ALLEN PRESS INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/163449]  |
| 专题 | 海洋研究所_海洋生物分类与系统演化实验室 |
| 通讯作者 | Lei, Yanli; Li, Tiegang |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao 266061, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.SOA, Inst Oceanog 1, Key Lab Marine Sedimentol & Environm Geol, Qingdao 266061, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lyu, Man,Lei, Yanli,Li, Tiegang. COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)[J]. JOURNAL OF FORAMINIFERAL RESEARCH,2018,48(3):186-192. |
| APA | Lyu, Man,Lei, Yanli,&Li, Tiegang.(2018).COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA).JOURNAL OF FORAMINIFERAL RESEARCH,48(3),186-192. |
| MLA | Lyu, Man,et al."COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)".JOURNAL OF FORAMINIFERAL RESEARCH 48.3(2018):186-192. |
入库方式： OAI收割
来源：海洋研究所
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