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Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects

文献类型：期刊论文

作者Zheng, Haifeng1,2; Chen, Si1; Wang, Xinan1; Xie, Jinrong1; Tian, Jie1,3,4; Wang, Fu1,2
刊名ANALYTICAL CHEMISTRY
出版日期2019-10-01
卷号91期号:19页码:12392-12398
ISSN号0003-2700
DOI10.1021/acs.analchem.9b02935
通讯作者Tian, Jie(jie.tian@ia.ac.cn) ; Wang, Fu(fwang@xidian.edu.cn)
英文摘要Pre-mRNA splicing in the information exchange from DNA to protein is a critical step in all eukaryotes. However, there is currently a lack of noninvasive approaches for monitoring mRNA splicing events in cells. In this study, we presented a genetically encoded bioluminescence reporter, Rluc-intron, for noninvasive real-time monitoring the premRNA splicing process in living cells and animals. It was designed by inserting a renilla luciferase (Rluc) gene into an intron sequence manipulated by RNA splicing modulator. We demonstrated that the splicing reporter Rluc-intron could provide real-time and quantitative information on the splicing activity responded to extracellular stimuli in living cells. In addition, Rluc-intron reporter is able to successfully quantify and image the pre-mRNA splicing in living mice in a noninvasive and longitudinal manner. This bioluminescence reporter provides the advantageous properties of systematic discovery of splicing modulators, which give the advances in pharmacogenomics and would produce new approach in the therapy of human diseases caused by splicing disorder.
WOS关键词ANTISENSE OLIGONUCLEOTIDES ; RETENTION
资助项目National Natural Science Foundation of China[81571721] ; National Natural Science Foundation of China[81772010] ; National Key Research and Development Program of China (973 Program)[2017YFA0205202]
WOS研究方向Chemistry
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000488993000036
资助机构National Natural Science Foundation of China ; National Key Research and Development Program of China (973 Program)
源URL[http://ir.ia.ac.cn/handle/173211/26444]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Tian, Jie; Wang, Fu
作者单位1.Xidian Univ, Sch Life Sci & Technol, Minist Educ, Engn Res Ctr Mol & Neuro Imaging, Xian 710071, Shaanxi, Peoples R China
2.Northwestern Polytech Univ, Inst Med Res, Xian 710072, Shaanxi, Peoples R China
3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
4.Beihang Univ, Sch Med, Beijing Adv Innovat Ctr Big Data Based Precis Med, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Haifeng,Chen, Si,Wang, Xinan,et al. Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects[J]. ANALYTICAL CHEMISTRY,2019,91(19):12392-12398.
APA Zheng, Haifeng,Chen, Si,Wang, Xinan,Xie, Jinrong,Tian, Jie,&Wang, Fu.(2019).Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects.ANALYTICAL CHEMISTRY,91(19),12392-12398.
MLA Zheng, Haifeng,et al."Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects".ANALYTICAL CHEMISTRY 91.19(2019):12392-12398.

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来源：自动化研究所

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