中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 数学与系统科学研究院
  3. 中国科学院数学与系统科学研究院
  4. 应用数学研究所
Model-based understanding of single-cell CRISPR screening

文献类型：期刊论文

作者Duan, Bin7,8; Zhou, Chi8; Zhu, Chengyu8; Yu, Yifei8; Li, Gaoyang5,6; Zhang, Shihua4; Zhang, Chao8; Ye, Xiangyun3; Ma, Hanhui2; Qu, Shen8
刊名NATURE COMMUNICATIONS
出版日期2019-05-20
卷号10页码:11
ISSN号2041-1723
DOI10.1038/s41467-019-10216-x
英文摘要The recently developed single-cell CRISPR screening techniques, independently termed Perturb-Seq, CRISP-seq, or CROP-seq, combine pooled CRISPR screening with single-cell RNA-seq to investigate functional CRISPR screening in a single-cell granularity. Here, we present MUSIC, an integrated pipeline for model-based understanding of single-cell CRISPR screening data. Comprehensive tests applied to all the publicly available data revealed that MUSIC accurately quantifies and prioritizes the individual gene perturbation effect on cell phenotypes with tolerance for the substantial noise that exists in such data analysis. MUSIC facilitates the single-cell CRISPR screening from three perspectives, i.e., prioritizing the gene perturbation effect as an overall perturbation effect, in a functional topic-specific way, and quantifying the relationships between different perturbations. In summary, MUSIC provides an effective and applicable solution to elucidate perturbation function and biologic circuits by a model-based quantitative analysis of single-cell-based CRISPR screening data.
资助项目National Key Research and Development Program of China[2017YFC0908500] ; National Key Research and Development Program of China[2016YFC1303205] ; National Natural Science Foundation of China[61572361] ; Shanghai Rising-Star Program[16QA1403900] ; Shanghai Natural Science Foundation Program[17ZR1449400] ; Fundamental Research Funds for the Central Universities
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000468274900002
出版者NATURE PUBLISHING GROUP
源URL[http://ir.amss.ac.cn/handle/2S8OKBNM/34711]  
专题应用数学研究所
通讯作者Wang, Ping; Sun, Shuyang; Liu, Qi
作者单位1.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Oral & Maxillofacial Head Neck Oncol, Coll Stomatol,Sch Med, Shanghai, Peoples R China
2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
3.Shanghai Jiao Tong Univ, Shanghai Chest Hosp, Shanghai, Peoples R China
4.Acad Math & Syst Sci, Inst Appl Math, Beijing, Peoples R China
5.Tongji Univ, Sch Med, Shanghai, Peoples R China
6.Tongji Univ, Shanghai Peoples Hosp 10, Ctr Canc, Shanghai, Peoples R China
7.Ninghai First Hosp, Dept Ophthalmol, Ninghai, Zhejiang, Peoples R China
8.Tongji Univ, Dept Endocrinol & Metab, Shanghai Peoples Hosp 10, Bioinformat Dept,Coll Life Sci, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Duan, Bin,Zhou, Chi,Zhu, Chengyu,et al. Model-based understanding of single-cell CRISPR screening[J]. NATURE COMMUNICATIONS,2019,10:11.
APA Duan, Bin.,Zhou, Chi.,Zhu, Chengyu.,Yu, Yifei.,Li, Gaoyang.,...&Liu, Qi.(2019).Model-based understanding of single-cell CRISPR screening.NATURE COMMUNICATIONS,10,11.
MLA Duan, Bin,et al."Model-based understanding of single-cell CRISPR screening".NATURE COMMUNICATIONS 10(2019):11.

入库方式： OAI收割

来源：数学与系统科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。