Differentiation of Dendrobium species used as "Huangcao Shihu" by rDNA ITS sequence analysis
文献类型：期刊论文
| 作者 | Xu, Hong; Wang, Zhengtao; Ding, Xiaoyu; Zhou, Kaiya; Xu, Luoshan |
| 刊名 | Planta medica
 |
| 出版日期 | 2006-01 |
| 卷号 | 72期号:1页码:89-92 |
| ISSN号 | 0032-0943 |
| DOI | 10.1055/s-2005-916228 |
| 文献子类 | Article |
| 英文摘要 | The genus Dendrobium Sw. is composed of 74 species and two varieties in China, and 32 species carry the name "Huangcao Shihu" on the herbal medicine market, making the identification of the origin of "Huangcao Shihu" difficult for consumers. Here, the ITS regions were sequenced and evaluated to differentiate the 18 Dendrobium species used as "Huangcao Shihu". Diversity in DNA sequences among various species was found with the inter-specific sequence divergence ranging from 3.2% to 37.9% in ITS1 and 5.0% to 26.6% in ITS2. Moreover, the variations within species were very low, ranging in sequence divergence from 0 to 3.0% in ITS1 and 0 to 4.0% in ITS2. Therefore, these species could be easily distinguished at the DNA level. Furthermore, based on the divergent ITS regions, five pairs of species-specific primers were designed and used for the rapid PCR identification of five Dendrobium species listed in the Chinese Pharmacopoeia. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266348]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China |
| 推荐引用方式 GB/T 7714 | Xu, Hong,Wang, Zhengtao,Ding, Xiaoyu,et al. Differentiation of Dendrobium species used as "Huangcao Shihu" by rDNA ITS sequence analysis[J]. Planta medica,2006,72(1):89-92. |
| APA | Xu, Hong,Wang, Zhengtao,Ding, Xiaoyu,Zhou, Kaiya,&Xu, Luoshan.(2006).Differentiation of Dendrobium species used as "Huangcao Shihu" by rDNA ITS sequence analysis.Planta medica,72(1),89-92. |
| MLA | Xu, Hong,et al."Differentiation of Dendrobium species used as "Huangcao Shihu" by rDNA ITS sequence analysis".Planta medica 72.1(2006):89-92. |
入库方式： OAI收割
来源：上海药物研究所
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