A quantitative ¹H nuclear magnetic resonance (qHNMR) method for assessing the purity of iridoids and secoiridoids
文献类型：期刊论文
| 作者 | Li, Zeyun2,3; Welbeck, Edward3; Yang, Li3; He, Chunyong3; Hu, Haijun3; Song, Ming3; Bi, Kaishun1; Wang, Zhengtao3 |
| 刊名 | Fitoterapia
 |
| 出版日期 | 2015-01 |
| 卷号 | 100页码:187-94 |
| ISSN号 | 1873-6971 |
| DOI | 10.1016/j.fitote.2014.12.001 |
| 文献子类 | Article |
| 英文摘要 | This paper utilized a quantitative (1)H nuclear magnetic resonance (qHNMR) method for assessing the purity of iridoids and secoiridoids. The method was fully validated, including specificity, linearity, accuracy, precision, reproducibility, and robustness. For optimization of experimental conditions, several experimental parameters were investigated, including relaxation delay (D1), scan numbers (NS) and power length (PL1). The quantification was based on the area ratios of H-3 from analytes relative to aromatic protons from 1,4-dinitrobenzene (internal standard) with methanol-d4 as solvent. Five iridoids and secoiridoids (sweroside, swertiamarin, gentiopicroside, geniposide, genipin) were analyzed. Furthermore, the results were validated by the high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) method. It can be concluded that the qHNMR method was simple, rapid, and accurate, providing a reliable and superior method for assessing the purity of iridoids and secoiridoids. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266376]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Bi, Kaishun; Wang, Zhengtao |
| 作者单位 | 1.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China 2.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 3.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; |
| 推荐引用方式 GB/T 7714 | Li, Zeyun,Welbeck, Edward,Yang, Li,et al. A quantitative ¹H nuclear magnetic resonance (qHNMR) method for assessing the purity of iridoids and secoiridoids[J]. Fitoterapia,2015,100:187-94. |
| APA | Li, Zeyun.,Welbeck, Edward.,Yang, Li.,He, Chunyong.,Hu, Haijun.,...&Wang, Zhengtao.(2015).A quantitative ¹H nuclear magnetic resonance (qHNMR) method for assessing the purity of iridoids and secoiridoids.Fitoterapia,100,187-94. |
| MLA | Li, Zeyun,et al."A quantitative ¹H nuclear magnetic resonance (qHNMR) method for assessing the purity of iridoids and secoiridoids".Fitoterapia 100(2015):187-94. |
入库方式： OAI收割
来源：上海药物研究所
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