Enhancement of vindoline production in suspension culture of the Catharanthus roseus cell line C20hi by light and methyl jasmonate elicitation
文献类型：期刊论文
| 作者 | He, Lihong; Yang, Li; Tan, Ronghui; Zhao, Shujuan; Hu, Zhibi |
| 刊名 | Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
 |
| 出版日期 | 2011 |
| 卷号 | 27期号:12页码:1243-8 |
| ISSN号 | 1348-2246 |
| 文献子类 | Article |
| 英文摘要 | The effects of light and methyl jasmonate (MJ) on the transcription of biosynthetic genes as well as the accumulation of vindoline and catharanthine in Catharanthus roseus C20hi cell suspensions were studied. t16h (the gene encoding tabersonine 16-hydroxylase) could be induced by light and MJ, whereas d4h (the gene encoding deacetoxyvindoline 4-hydroxylase) could only be induced by light. Quantification by UPLC-MS showed that light significantly increased vindoline production in C20hi cells by about 0.49 - 5.51-fold more than that in controls, with the highest yield being 75.3 ng/g of dry weight. The biosynthesis of vindoline was further enhanced by combining MJ with light. The accumulation of catharanthine was not improved by either light or MJ elicitation. These results suggested that light and MJ could promote vindoline, but not catharanthine accumulation in C20hi cells. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266382]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
| 推荐引用方式 GB/T 7714 | He, Lihong,Yang, Li,Tan, Ronghui,et al. Enhancement of vindoline production in suspension culture of the Catharanthus roseus cell line C20hi by light and methyl jasmonate elicitation[J]. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,2011,27(12):1243-8. |
| APA | He, Lihong,Yang, Li,Tan, Ronghui,Zhao, Shujuan,&Hu, Zhibi.(2011).Enhancement of vindoline production in suspension culture of the Catharanthus roseus cell line C20hi by light and methyl jasmonate elicitation.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,27(12),1243-8. |
| MLA | He, Lihong,et al."Enhancement of vindoline production in suspension culture of the Catharanthus roseus cell line C20hi by light and methyl jasmonate elicitation".Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 27.12(2011):1243-8. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。