Establishment and application of a new HPLC qualitative and quantitative assay for Gentiana Macrophyllae Radix based on characteristic constituents of anofinic acid and its derivatives
文献类型：期刊论文
| 作者 | Ding, Wenzheng3,4,5,6; Wang, Hanxue2; Zhou, Quan3,4,5,6; Wu, Jinrong1; Cheng, Xuemei3,4,5,6; Wang, Changhong3,4,5,6 |
| 刊名 | Biomedical chromatography : BMC
 |
| 出版日期 | 2018-11 |
| 卷号 | 32期号:11页码:e4341 |
| ISSN号 | 1099-0801 |
| DOI | 10.1002/bmc.4341 |
| 文献子类 | Article |
| 英文摘要 | Gentiana Macrophylla Radix (GMR) is officially used as traditional Chinese medicine, but easily confused with Gentianae Radix et Rhizoma (GRR) and adulterants. This study aimed to establish an HPLC method for qualitative and quantitative analysis of GMR based on characteristic components, anofinic acid and its derivatives. HPLC analysis was performed on a C column with gradient elution using acetonitrile and 0.1% phosphoric acid as mobile phase, and detected at 240 nm by conventional methodology validation. For fingerprint analysis, RSDs of relative retention times and relative peak areas of the characteristic peaks were within 0-1.10 and 0-4.08%, respectively. For determination of 2-methoxyanofinic acid, the calibration curve showed good linearity (R > 0.9999) within the test range. The RSDs of precision, repeatability and stability test did not exceed 2.46, 0.83 and 1.11%, respectively. The average recoveries were between 95.08 and 103.05% with RSDs ≤2.29%. The results showed that there was no significant difference among the four species of GMR, but there were significant differences among GMR, GRR and spurious breeds by principal component analysis and hierarchical cluster analysis. Anofinic acid and its derivatives, as the characteristic markers, could be used for the identification and quality control of GMR. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266385]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China 2.Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 3.Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai, China; 4.Shanghai Key Laboratory of Compound Chinese Medicines; 5.The MOE Key Laboratory for Standardization of Chinese Medicines; 6.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; |
| 推荐引用方式 GB/T 7714 | Ding, Wenzheng,Wang, Hanxue,Zhou, Quan,et al. Establishment and application of a new HPLC qualitative and quantitative assay for Gentiana Macrophyllae Radix based on characteristic constituents of anofinic acid and its derivatives[J]. Biomedical chromatography : BMC,2018,32(11):e4341. |
| APA | Ding, Wenzheng,Wang, Hanxue,Zhou, Quan,Wu, Jinrong,Cheng, Xuemei,&Wang, Changhong.(2018).Establishment and application of a new HPLC qualitative and quantitative assay for Gentiana Macrophyllae Radix based on characteristic constituents of anofinic acid and its derivatives.Biomedical chromatography : BMC,32(11),e4341. |
| MLA | Ding, Wenzheng,et al."Establishment and application of a new HPLC qualitative and quantitative assay for Gentiana Macrophyllae Radix based on characteristic constituents of anofinic acid and its derivatives".Biomedical chromatography : BMC 32.11(2018):e4341. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。