α-asarone from Acorus gramineus alleviates epilepsy by modulating A-type GABA receptors
文献类型：期刊论文
| 作者 | Huang, Chen; Li, Wei-Guang; Zhang, Xiao-Bing; Wang, Li; Xu, Tian-Le; Wu, Dazheng; Li, Yong |
| 刊名 | Neuropharmacology
 |
| 出版日期 | 2013-02 |
| 卷号 | 65页码:1-11 |
| ISSN号 | 1873-7064 |
| DOI | 10.1016/j.neuropharm.2012.09.001 |
| 文献子类 | Article |
| 英文摘要 | Alpha (α)-asarone is a major effective compound isolated from the Chinese medicinal herb Acorus gramineus, which is widely used in clinical practice as an antiepileptic drug; however, its mechanism of action remains unclear. In this study, we have characterized the action of α-asarone on the excitability of rat hippocampal neurons in culture and on the epileptic activity induced by pentylenetetrazole or kainate injection in vivo. Under cell-attached configuration, the firing rate of spontaneous spiking was inhibited by application of α-asarone, which was maintained in the Mg(2+)-free solution. Under whole-cell configuration, α-asarone induced inward currents in a concentration-dependent manner with an EC(50) of 248 ± 33 μM, which was inhibited by a GABA(A) receptor blocker picotoxin and a competitive GABA(A) receptor antagonist bicuculline but not a specific glycine receptor inhibitor strychnine. Measurement of tonic GABA currents and miniature spontaneous inhibitory postsynaptic currents indicated that α-asarone enhanced tonic GABAergic inhibition while left phasic GABAergic inhibition unaffected. In both pentylenetetrazole and kainate seizure models, α-asarone suppressed epileptic activity of mice by prolonging the latency to clonic and tonic seizures and reducing the mortality as well as the susceptibility to seizure in vivo presumably dependent on the activation of GABA(A) receptors. In summary, our results suggest that α-asarone inhibits the activity of hippocampal neurons and produces antiepileptic effect in central nervous system through enhancing tonic GABAergic inhibition. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266389]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China |
| 推荐引用方式 GB/T 7714 | Huang, Chen,Li, Wei-Guang,Zhang, Xiao-Bing,et al. α-asarone from Acorus gramineus alleviates epilepsy by modulating A-type GABA receptors[J]. Neuropharmacology,2013,65:1-11. |
| APA | Huang, Chen.,Li, Wei-Guang.,Zhang, Xiao-Bing.,Wang, Li.,Xu, Tian-Le.,...&Li, Yong.(2013).α-asarone from Acorus gramineus alleviates epilepsy by modulating A-type GABA receptors.Neuropharmacology,65,1-11. |
| MLA | Huang, Chen,et al."α-asarone from Acorus gramineus alleviates epilepsy by modulating A-type GABA receptors".Neuropharmacology 65(2013):1-11. |
入库方式： OAI收割
来源：上海药物研究所
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