Comparison of isorhamnetin absorption properties in total flavones of Hippophae rhamnoides L. with its pure form in a Caco-2 cell model mediated by multidrug resistance-associated protein
文献类型：期刊论文
| 作者 | Xie, Yan4,5; Duan, Jingze3,5; Fu, Qingxue3,5; Xia, Mengxin5; Zhang, Lei5; Li, Guowen2; Wu, Tao1; Ji, Guang4 |
| 刊名 | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
 |
| 出版日期 | 2015-06-20 |
| 卷号 | 73页码:1-8 |
| ISSN号 | 1879-0720 |
| DOI | 10.1016/j.ejps.2015.03.008 |
| 文献子类 | Article |
| 英文摘要 | Total flavones of Hippophae rhamnoides L. (TFH) are extracted from the widely distributed thorny bush Sea buckthorn (Hippophae rhamnoides L.). Isorhamnetin (IS) is one of the representative ingredients in TFH. In this study, the absorption properties of IS in TFH and its pure form were compared through transepithelial transport and cellular uptake experiments in a Caco-2 cell model. Our results show that the absorption properties of IS in TFH and its pure form were remarkably different: (1) Both PappAB and PappBA of IS in TFH were dramatically increased compared with those of IS pure form; consequently, its Pratio was 2.3-fold higher than that of IS; (2) Both the accumulation and efflux of IS in TFH were significantly enhanced compared with the single compound. One likely reason for these differences is that the multiple components in TFH significantly down regulated the mRNA expression level of MRP2, which lead to a decrease in the protein level of MRP2, based on western blotting and RT-PCR assays. This study highlights the significant differences in the absorption properties of flavonoid components in different forms and the potential multi-component interactions in TFH. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266406]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Xie, Yan; Ji, Guang |
| 作者单位 | 1.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 2.Pharmacy Department, Shanghai TCM-integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China; 3.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 4.Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China; 5.Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; |
| 推荐引用方式 GB/T 7714 | Xie, Yan,Duan, Jingze,Fu, Qingxue,et al. Comparison of isorhamnetin absorption properties in total flavones of Hippophae rhamnoides L. with its pure form in a Caco-2 cell model mediated by multidrug resistance-associated protein[J]. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences,2015,73:1-8. |
| APA | Xie, Yan.,Duan, Jingze.,Fu, Qingxue.,Xia, Mengxin.,Zhang, Lei.,...&Ji, Guang.(2015).Comparison of isorhamnetin absorption properties in total flavones of Hippophae rhamnoides L. with its pure form in a Caco-2 cell model mediated by multidrug resistance-associated protein.European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences,73,1-8. |
| MLA | Xie, Yan,et al."Comparison of isorhamnetin absorption properties in total flavones of Hippophae rhamnoides L. with its pure form in a Caco-2 cell model mediated by multidrug resistance-associated protein".European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 73(2015):1-8. |
入库方式： OAI收割
来源：上海药物研究所
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