Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide
文献类型：期刊论文
| 作者 | Liu, Hongshuai; Huang, Fei; Wu, Hui; Zhang, Beibei; Shi, Hailian; Wu, Xiaojun; Hu, Zhibi |
| 刊名 | International journal of molecular medicine
 |
| 出版日期 | 2017-10 |
| 卷号 | 40期号:4页码:1270-1276 |
| ISSN号 | 1791-244X |
| DOI | 10.3892/ijmm.2017.3114 |
| 文献子类 | Article |
| 英文摘要 | The excessive activation of microglia in many neurodegenerative diseases is detrimental to neuronal survival. Isoastragaloside I (ISO I) is a natural saponin molecule found within the roots of Astragalus membranaceus, a famous traditional Chinese medicine. In the present study, the anti‑inflammatory effects and the mechanisms of action of ISO I on activated BV-2 cells stimulated with lipopolysaccharide (LPS) were investigated. ISO I dose‑dependently inhibited the excessive release of nitric oxide (NO) and tumor necrosis factor (TNF)-α in the LPS-stimulated BV-2 cells. Moreover, it decreased the production of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), and mitigated the gene expression of interleukin (IL)-1β, TNF-α and iNOS induced by LPS. Further experiments revealed that ISO I decreased the phosphorylation levels of nuclear factor-κB (NF-κB), and suppressed its nuclear translocation and transactivation activity. In addition, it inhibited the activation of signaling pathway molecules, such as PI3K, Akt and mitogen-activated protein kinases (MAPKs). Taken together, our findings suggest that ISO I prevents LPS-induced microglial activation probably by inhibiting the activation of the NF-κB via PI3K/Akt and MAPK signaling pathways, indicating its therapeutic potential for neurological diseases relevant to neuroinflammation. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266423]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Shanghai Key Laboratory of Compound Chinese Medicines, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China |
| 推荐引用方式 GB/T 7714 | Liu, Hongshuai,Huang, Fei,Wu, Hui,et al. Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide[J]. International journal of molecular medicine,2017,40(4):1270-1276. |
| APA | Liu, Hongshuai.,Huang, Fei.,Wu, Hui.,Zhang, Beibei.,Shi, Hailian.,...&Hu, Zhibi.(2017).Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide.International journal of molecular medicine,40(4),1270-1276. |
| MLA | Liu, Hongshuai,et al."Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide".International journal of molecular medicine 40.4(2017):1270-1276. |
入库方式： OAI收割
来源：上海药物研究所
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