The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism
文献类型：期刊论文
| 作者 | Fu, Qingxue1,2; Wang, Huizhen2; Xia, Mengxin2; Deng, Bing2; Shen, Hongyi2; Ji, Guang3; Li, Guowen4; Xie, Yan2,3 |
| 刊名 | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 80页码:1-8 |
| ISSN号 | 1879-0720 |
| DOI | 10.1016/j.ejps.2015.09.009 |
| 文献子类 | Article |
| 英文摘要 | This study investigated the effect of phytic acid (IP6), a potential absorption enhancer of flavonoid components, on tight junction (TJ) integrity in Caco-2 cell monolayers and its possible mechanisms. Transepithelial electrical resistance (TEER) across the monolayers decreased rapidly, and the flux of fluorescein sodium (a paracellular marker) increased after treating with IP6 in a concentration-dependent manner. Confocal microscopy results showed that IP6 produced a concentration-dependent attenuation in the distribution of occludin, ZO-1, and claudin-1. Immunoblot analysis revealed that IP6 could down-regulate the expression level of these TJ proteins, which resulted in the opening of TJ. Additionally, the divalent cations Ca(2+) and Mg(2+) influenced the IP6-induced distribution of occludin, ZO-1, and claudin-1 in different directions, which enhanced barrier function. In conclusion, IP6 can decrease the integrity of Caco-2 cell monolayers by modulating the TJ proteins' localization and down-regulating the expression levels of TJ proteins including claudin-1, occludin, and ZO-1; the reduction effects of divalent cations such as Ca(2+) and Mg(2+) on the regulation of TJ induced by IP6 should be addressed. The present work will offer some useful guidance for the application of IP6 in drug delivery area. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266428]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Guowen; Xie, Yan |
| 作者单位 | 1.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2.Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 3.Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China; 4.Pharmacy Department, Shanghai TCM-integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China |
| 推荐引用方式 GB/T 7714 | Fu, Qingxue,Wang, Huizhen,Xia, Mengxin,et al. The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism[J]. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences,2015,80:1-8. |
| APA | Fu, Qingxue.,Wang, Huizhen.,Xia, Mengxin.,Deng, Bing.,Shen, Hongyi.,...&Xie, Yan.(2015).The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism.European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences,80,1-8. |
| MLA | Fu, Qingxue,et al."The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism".European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 80(2015):1-8. |
入库方式： OAI收割
来源：上海药物研究所
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