Simultaneous analysis of trans- and cis-isomers of 2-glucosyloxycinnamic acids and coumarin derivatives in Dendrobium thyrsiflorum by high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-tandem mass spectrometry (MS)
文献类型：期刊论文
| 作者 | Zhang, Guangnong; Zhang, Fang; Yang, Li; Zhu, Enyuan; Wang, Zhengtao; Xu, Luoshan; Hu, Zhibi |
| 刊名 | Analytica chimica acta
 |
| 出版日期 | 2006-06-30 |
| 卷号 | 571期号:1页码:17-24 |
| ISSN号 | 1873-4324 |
| DOI | 10.1016/j.aca.2006.04.062 |
| 文献子类 | Article |
| 英文摘要 | A novel method has been developed for simultaneous analysis for three pairs of trans- and cis-isomers of 2-glucosyloxycinnamic acids, along with their biogenic metabolites (three coumarin derivatives including scopoletin, scoparone and ayapin) in a Chinese medicinal herb Dendrobium thyrsiflorum by using high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-tandem mass spectrometry (MS). The method was carried out by using a Polaris C18 column with a gradient solvent system of 0.5% acetic acid aqueous solution-acetonitrile. Seven target analytes including isodensifloside, isothyrsifloside, densifloside, thyrsifloside, scoparone, ayapin and scopoletin were exclusively identified by comparing their retention behaviors, UV and MS spectra with the authentic standards, and their contents in D. thyrsiflorum were simultaneous determined by employing UV detection at 342 nm. In addition, another pair of isomers of 2-glucosyloxycinnamic acids was putatively elucidated mainly based on the MS fragmentation. The method was validated and found to be satisfactorily linear, selective and robust. Recoveries ranged from 95.56 to 97.94% for all compounds at three different spiking levels. The limits of detection (LOD) and quantitation (LOQ) ranged, respectively, from 0.02 to 0.13 microg mL(-1) and 0.07 to 0.39 microg mL(-1) depending on various compounds. The established quality evaluation method was successfully used for evaluating the quality of D. thyrsiflorum samples of different organs and collections. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266472]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cai Lun Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China |
| 推荐引用方式 GB/T 7714 | Zhang, Guangnong,Zhang, Fang,Yang, Li,et al. Simultaneous analysis of trans- and cis-isomers of 2-glucosyloxycinnamic acids and coumarin derivatives in Dendrobium thyrsiflorum by high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-tandem mass spectrometry (MS)[J]. Analytica chimica acta,2006,571(1):17-24. |
| APA | Zhang, Guangnong.,Zhang, Fang.,Yang, Li.,Zhu, Enyuan.,Wang, Zhengtao.,...&Hu, Zhibi.(2006).Simultaneous analysis of trans- and cis-isomers of 2-glucosyloxycinnamic acids and coumarin derivatives in Dendrobium thyrsiflorum by high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-tandem mass spectrometry (MS).Analytica chimica acta,571(1),17-24. |
| MLA | Zhang, Guangnong,et al."Simultaneous analysis of trans- and cis-isomers of 2-glucosyloxycinnamic acids and coumarin derivatives in Dendrobium thyrsiflorum by high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-tandem mass spectrometry (MS)".Analytica chimica acta 571.1(2006):17-24. |
入库方式： OAI收割
来源：上海药物研究所
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