Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata
文献类型：期刊论文
| 作者 | Wang, Hongwei1; Li, Ning1; Zhu, Chao1; Shi, Songshan1; Jin, Hong2; Wang, Shunchun1 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2017-11-18 |
| 卷号 | 493期号:2页码:887-893 |
| ISSN号 | 1090-2104 |
| DOI | 10.1016/j.bbrc.2017.09.126 |
| 文献子类 | Article |
| 英文摘要 | Complement-mediated inflammation and tissue damage is an important drive to pathology in autoimmune diseases, targeting inhibit the complement activation is promising treatment strategy for these diseases. We performed anticomplement activity-guided fractionation of the water extract of Eclipta prostrata by ion-exchange and size-exclusion chromatography, yielding two bioactive polysaccharides EAP20-1 and EAP20-2. The molecular weights of EAP20-1 and EAP20-2 were respectively calculated to be 5.2 kDa and 6.3 kDa by HPGPC, both polysaccharides was composed by d-Gal, l-Glc, and Ara at different ratios of 7.3:2.7:1 and 7.6:3.1:1. In addition, the main linkage types of EAP20-1 and EAP20-2 were β-1,4-Gal, β-1,6-Gal and α-1,4,6-Glc according to methylation analyses. EAP20-1 and EAP20-2 exhibited significant inhibitory effect on the complement activation through both classical and alternative pathways and with no influence on the coagulation system. Preliminary mechanism study indicated that both EAP20-1 and EAP20-2 inhibited the activation of the complement system by interacting with C1q, C1r, C1s, C2, C4, C5, C7, and C9 components. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266475]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wang, Shunchun |
| 作者单位 | 1.The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China; 2.The Qingdao Environmental Monitoring Station, Qingdao 266003, China |
| 推荐引用方式 GB/T 7714 | Wang, Hongwei,Li, Ning,Zhu, Chao,et al. Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata[J]. Biochemical and biophysical research communications,2017,493(2):887-893. |
| APA | Wang, Hongwei,Li, Ning,Zhu, Chao,Shi, Songshan,Jin, Hong,&Wang, Shunchun.(2017).Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata.Biochemical and biophysical research communications,493(2),887-893. |
| MLA | Wang, Hongwei,et al."Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata".Biochemical and biophysical research communications 493.2(2017):887-893. |
入库方式： OAI收割
来源：上海药物研究所
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