The involvement of Nrf2 antioxidant signalling pathway in the protection of monocrotaline-induced hepatic sinusoidal obstruction syndrome in rats by (+)-catechin hydrate
文献类型：期刊论文
| 作者 | Jing, Xiaoqi1; Zhang, Jiaqi1; Huang, Zhenlin1; Sheng, Yuchen2; Ji, Lili1 |
| 刊名 | Free radical research
 |
| 出版日期 | 2018-04 |
| 卷号 | 52期号:4页码:402-414 |
| ISSN号 | 1029-2470 |
| DOI | 10.1080/10715762.2018.1437914 |
| 文献子类 | Article |
| 英文摘要 | Hepatic sinusoidal obstruction syndrome (HSOS) is a rare and life-threatening liver disease. (+)-Catechin is a natural dietary flavonol with high antioxidant capacity. This study aims to investigate the involvement of nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant signalling pathway in the protection of (+)-catechin hydrate (CAT) against monocrotaline (MCT)-induced HSOS. Results of serum alanine/aspartate aminotransferases (ALT/AST) activities, total bilirubin (TBil) and bile acids (TBA) amounts, liver histological observation, scanning electron microscope evaluation, and hepatic metalloproteinase-9 (MMP-9) expression all demonstrated the protection of CAT against MCT-induced HSOS in rats. CAT attenuated MCT-induced liver oxidative injury in rats and the formation of cellular reactive oxygen species (ROS) in human hepatic sinusoidal endothelial cells (HHSECs). CAT-enhanced Nrf2 nuclear translocation in livers from MCT-treated rats and in HHSECs treated with MCT, and further increased the expression of Nrf2-dependent genes including catalytic or modify subunit of glutamate-cysteine ligase (GCLC/GCLM), haem oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1). Moreover, GCL inhibitor -buthionine-(S, R)-sulfoximine (BSO), NQO1 inhibitor diminutol (Dim), and HO-1 inhibitor zinc protoporphyrin (ZnPP) all abrogated CAT-provided the protection against MCT-induced cytotoxicity in HHSECs. The results of molecular docking analysis indicated the potential interaction of CAT with the Nrf2-binding site in kelch-like ECH-associated protein-1 (Keap1) protein. In summary, this study demonstrated the critical involvement of Nrf2 antioxidant signalling pathway in CAT-provided the protection against MCT-induced HSOS. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266483]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.a MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines , Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine , Shanghai , China; 2.b Center for Drug Safety Evaluation and Research, Shanghai University of Traditional Chinese Medicine , Shanghai , China |
| 推荐引用方式 GB/T 7714 | Jing, Xiaoqi,Zhang, Jiaqi,Huang, Zhenlin,et al. The involvement of Nrf2 antioxidant signalling pathway in the protection of monocrotaline-induced hepatic sinusoidal obstruction syndrome in rats by (+)-catechin hydrate[J]. Free radical research,2018,52(4):402-414. |
| APA | Jing, Xiaoqi,Zhang, Jiaqi,Huang, Zhenlin,Sheng, Yuchen,&Ji, Lili.(2018).The involvement of Nrf2 antioxidant signalling pathway in the protection of monocrotaline-induced hepatic sinusoidal obstruction syndrome in rats by (+)-catechin hydrate.Free radical research,52(4),402-414. |
| MLA | Jing, Xiaoqi,et al."The involvement of Nrf2 antioxidant signalling pathway in the protection of monocrotaline-induced hepatic sinusoidal obstruction syndrome in rats by (+)-catechin hydrate".Free radical research 52.4(2018):402-414. |
入库方式： OAI收割
来源：上海药物研究所
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