Chemical fingerprint and simultaneous determination of alkaloids and flavonoids in aerial parts of genus Peganum indigenous to China based on HPLC-UV: application of analysis on secondary metabolites accumulation
文献类型：期刊论文
| 作者 | Wen, Fangfang; Cheng, Xuemei; Liu, Wei; Xuan, Min; Zhang, Lei; Zhao, Xin; Shan, Meng; Li, Yan; Teng, Liang; Wang, Zhengtao |
| 刊名 | Biomedical chromatography : BMC
 |
| 出版日期 | 2014-12 |
| 卷号 | 28期号:12页码:1763-73 |
| ISSN号 | 1099-0801 |
| DOI | 10.1002/bmc.3218 |
| 文献子类 | Article |
| 英文摘要 | The aerial parts of genus Peganum are officially used in traditional Chinese medicine. The paper aims to establish a high-performance liquid chromatography (HPLC) method for fingerprint analysis and simultaneous determination of three alkaloids and two flavonoids in aerial parts of genus Peganum, and to analyze accumulative difference of secondary metabolites in inter-species, individuals of plants, inter-/intra-population and from different growing seasons. HPLC analysis was performed on a C18 column with gradient elution using 0.1% trifloroacetic acid and acetonitrile as mobile phase and detected at 265 nm, by conventional methodology validation. For fingerprint analysis, the RSDs of relative retention time and relative peak area of the characteristic peaks were within 0.07-0.78 and 0.94-9.09%, respectively. For simultaneous determination of vasicine, harmaline, harmine, deacetylpeganetin and peganetin, all calibration curves showed good linearity (r > 0.9990) within the test range. The relative standard deviations of precision, repeatability and stability test did not exceed 2.37, 2.68 and 2.67%, respectively. The average recoveries for the five analytes were between 96.47 and 101.20%. HPLC fingerprints play a minor role in authenticating and differentiating the herbs of different species of genus Peganum. However, the secondary metabolites levels of alkaloids and flavonoids in aerial parts of genus Peganum rely on species-, habitat-, and growth season-dependent accumulation. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266507]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai R&D Centre for Standardization of Chinese Medicines, 1200 Cailun Road, Shanghai, 201203, People's Republic of China |
| 推荐引用方式 GB/T 7714 | Wen, Fangfang,Cheng, Xuemei,Liu, Wei,et al. Chemical fingerprint and simultaneous determination of alkaloids and flavonoids in aerial parts of genus Peganum indigenous to China based on HPLC-UV: application of analysis on secondary metabolites accumulation[J]. Biomedical chromatography : BMC,2014,28(12):1763-73. |
| APA | Wen, Fangfang.,Cheng, Xuemei.,Liu, Wei.,Xuan, Min.,Zhang, Lei.,...&Wang, Changhong.(2014).Chemical fingerprint and simultaneous determination of alkaloids and flavonoids in aerial parts of genus Peganum indigenous to China based on HPLC-UV: application of analysis on secondary metabolites accumulation.Biomedical chromatography : BMC,28(12),1763-73. |
| MLA | Wen, Fangfang,et al."Chemical fingerprint and simultaneous determination of alkaloids and flavonoids in aerial parts of genus Peganum indigenous to China based on HPLC-UV: application of analysis on secondary metabolites accumulation".Biomedical chromatography : BMC 28.12(2014):1763-73. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。