Complete chloroplast genome sequence of poisonous and medicinal plant Datura stramonium: organizations and implications for genetic engineering
文献类型：期刊论文
| 作者 | Yang, Yang1; Dang, Yuanye1; Yuanye, Dang; Li, Qing3; Qing, Li; Lu, Jinjian1; Jinjian, Lu; Li, Xiwen1,2; Xiwen, Li; Wang, Yitao1 |
| 刊名 | PloS one
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:11页码:e110656 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0110656 |
| 文献子类 | Article |
| 英文摘要 | Datura stramonium is a widely used poisonous plant with great medicinal and economic value. Its chloroplast (cp) genome is 155,871 bp in length with a typical quadripartite structure of the large (LSC, 86,302 bp) and small (SSC, 18,367 bp) single-copy regions, separated by a pair of inverted repeats (IRs, 25,601 bp). The genome contains 113 unique genes, including 80 protein-coding genes, 29 tRNAs and four rRNAs. A total of 11 forward, 9 palindromic and 13 tandem repeats were detected in the D. stramonium cp genome. Most simple sequence repeats (SSR) are AT-rich and are less abundant in coding regions than in non-coding regions. Both SSRs and GC content were unevenly distributed in the entire cp genome. All preferred synonymous codons were found to use A/T ending codons. The difference in GC contents of entire genomes and of the three-codon positions suggests that the D. stramonium cp genome might possess different genomic organization, in part due to different mutational pressures. The five most divergent coding regions and four non-coding regions (trnH-psbA, rps4-trnS, ndhD-ccsA, and ndhI-ndhG) were identified using whole plastome alignment, which can be used to develop molecular markers for phylogenetics and barcoding studies within the Solanaceae. Phylogenetic analysis based on 68 protein-coding genes supported Datura as a sister to Solanum. This study provides valuable information for phylogenetic and cp genetic engineering studies of this poisonous and medicinal plant. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266509]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China; 2.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 3.Department of Pharmacy, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China; |
| 推荐引用方式 GB/T 7714 | Yang, Yang,Dang, Yuanye,Yuanye, Dang,et al. Complete chloroplast genome sequence of poisonous and medicinal plant Datura stramonium: organizations and implications for genetic engineering[J]. PloS one,2014,9(11):e110656. |
| APA | Yang, Yang.,Dang, Yuanye.,Yuanye, Dang.,Li, Qing.,Qing, Li.,...&Yitao, Wang.(2014).Complete chloroplast genome sequence of poisonous and medicinal plant Datura stramonium: organizations and implications for genetic engineering.PloS one,9(11),e110656. |
| MLA | Yang, Yang,et al."Complete chloroplast genome sequence of poisonous and medicinal plant Datura stramonium: organizations and implications for genetic engineering".PloS one 9.11(2014):e110656. |
入库方式： OAI收割
来源：上海药物研究所
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