Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system
文献类型：期刊论文
| 作者 | Lv, Xiaocheng5,6; Chen, Dandan1,7; Yang, Liecheng1,7; Zhu, Ning5,6; Li, Jingling1,7; Zhao, Jian2 ; Hu, Zhibi5,6; Wang, Fu-Jun4,5,6; Zhang, Leshuai W3,4,7
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| 刊名 | International journal of biological macromolecules
 |
| 出版日期 | 2016-05 |
| 卷号 | 86页码:28-42 |
| ISSN号 | 1879-0003 |
| DOI | 10.1016/j.ijbiomac.2016.01.048 |
| 文献子类 | Article |
| 英文摘要 | In this study, polysaccharides were isolated from Astragalus membranaceus, Ganoderma lucidum and Radix ophiopogonis and named APSII, GLPII and OGPII for comparison of their immunoactivities. MTT assay indicated that these polysaccharides increased the metabolic activity of Raw264.7 macrophages and induced cell differentiation to dendritic like cells. High content screening and mathematical modeling were used to quantify the cell irregularity, a hallmark of cell differentiation by polysaccharides. The results showed that GLPII increased cell irregularity, but APSII and OGPII had slightly less effects. Imaging analysis also revealed that polysaccharides inhibited cell proliferation while inducing the cell differentiation. In addition, APSII and GLPII but not OGPII induced NO production and enhanced cell phagocytic ability. Interestingly, inducible nitric oxide synthase inhibitor blocked polysaccharide-enhanced phagocytosis, indicating NO production is crucial for macrophages to acquire phagocytic ability, which was further confirmed by correlation studies. APSII and GLPII significantly promoted the maturation of macrophages by the increase in the expression of MHCII, CD40, CD80 and CD86, while OGPII had less effects. In summary, we have suggested a practical and economical method to quantify macrophage differentiation (irregularity) induced by polysaccharides for quality assurance and have found the role of NO production on macrophage phagocytic ability. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266534]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wang, Fu-Jun; Zhang, Leshuai W |
| 作者单位 | 1.Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, 215123, China; 2.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 3.Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, 215123, China 4.Zhejiang Reachall Pharmaceutical Co., Ltd., Zhejiang 322100, China; 5.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 6.Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai 201210, China; 7.School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, 215123, China; |
| 推荐引用方式 GB/T 7714 | Lv, Xiaocheng,Chen, Dandan,Yang, Liecheng,et al. Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system[J]. International journal of biological macromolecules,2016,86:28-42. |
| APA | Lv, Xiaocheng.,Chen, Dandan.,Yang, Liecheng.,Zhu, Ning.,Li, Jingling.,...&Zhang, Leshuai W.(2016).Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system.International journal of biological macromolecules,86,28-42. |
| MLA | Lv, Xiaocheng,et al."Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system".International journal of biological macromolecules 86(2016):28-42. |
入库方式： OAI收割
来源：上海药物研究所
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