Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli
文献类型：期刊论文
| 作者 | Shi, Jie; Wang, Jie; Yu, Lu; Yang, Li; Zhao, Shujuan; Wang, Zhengtao |
| 刊名 | Journal of industrial microbiology & biotechnology
 |
| 出版日期 | 2017-07 |
| 卷号 | 44期号:7页码:1073-1082 |
| ISSN号 | 1476-5535 |
| DOI | 10.1007/s10295-017-1935-y |
| 文献子类 | Article |
| 英文摘要 | Bear bile powder is a precious medicinal material. It is characterized by high content of tauroursodeoxycholic acid (TUDCA) at a ratio of 1.0-1.5 to taurochenodeoxycholic acid (TCDCA). Here, we reported the biotransformation of tauroursodeoxycholic acid (TUDCA) through Escherichia coli engineered with a two-step mimic biosynthetic pathway of TUDCA from taurochenodeoxycholic acid (TCDCA). Two 7α-hydroxysteroid dehydrogenase (7α-HSDH) and two 7β-hydroxysteroid dehydrogenase (7β-HSDH) genes (named as α, α, β, and β) were selected and synthesized to create four pathway variants using ePathBrick. All could convert TCDCA to TUDCA and the one harboring α and β (pαβ) showed the strongest capability. Utilizing the oxidative and reductive properties of 7α- and 7β-HSDH, an ideal balance between TUDCA and TCDCA was established by optimizing the fermentation conditions. By applying the optimal condition, E. coli containing pαβ (BL-pαβ) produced up to 1.61 ± 0.13 g/L of TUDCA from 3.23 g/L of TCDCA at a ratio of 1.3 to TCDCA. This study provides a potential approach for bear bile substitute production from cheap and readily available chicken bile. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266535]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhao, Shujuan; Wang, Zhengtao |
| 作者单位 | The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China |
| 推荐引用方式 GB/T 7714 | Shi, Jie,Wang, Jie,Yu, Lu,et al. Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli[J]. Journal of industrial microbiology & biotechnology,2017,44(7):1073-1082. |
| APA | Shi, Jie,Wang, Jie,Yu, Lu,Yang, Li,Zhao, Shujuan,&Wang, Zhengtao.(2017).Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli.Journal of industrial microbiology & biotechnology,44(7),1073-1082. |
| MLA | Shi, Jie,et al."Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli".Journal of industrial microbiology & biotechnology 44.7(2017):1073-1082. |
入库方式： OAI收割
来源：上海药物研究所
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