中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study

文献类型:期刊论文

作者Zhou, Dandan; Tong, Ling; Wan, Meixu; Wang, Gangli; Ye, Zhengliang; Wang, Zhengtao; Lin, Ruichao
刊名Biomedical chromatography : BMC
出版日期2011-06
卷号25期号:6页码:720-6
ISSN号1099-0801
DOI10.1002/bmc.1508
文献子类Article
英文摘要A sensitive and reliable LC-ESI-MS method for simultaneous determination of nine ginsenosides (Rh(1), Rg(2), Rg(1), Rf, Re, Rd, Rc, Rb(2) and Rb(1)) in rat plasma was developed and validated using saikosaponin A as an internal standard. The samples were extracted by solid-phase extraction. Chromatographic separation was carried out on a Hypersil Gold C(18) column (100 × 2.1 mm, 5 µm) by stepwise gradient elution with water (0.1% formic acid, v/v) and acetonitrile as the mobile phase. Detection was determined by selective ion monitoring mode using electrospray ionization in the negative ion mode. Good linearity over the investigated concentration ranges was observed with the values of r higher than 0.9900. The intra- and inter-day precisions were all no more than 15% and the average recoveries varied from 71.8 to 91.7%. This quantitative measurement was successfully applied to a pharmacokinetic study of Yi-Qi-Fu-Mai injection.
语种英语
源URL[http://119.78.100.183/handle/2S10ELR8/266539]  
专题中国科学院上海药物研究所
作者单位Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, People's Republic of China
推荐引用方式
GB/T 7714
Zhou, Dandan,Tong, Ling,Wan, Meixu,et al. An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study[J]. Biomedical chromatography : BMC,2011,25(6):720-6.
APA Zhou, Dandan.,Tong, Ling.,Wan, Meixu.,Wang, Gangli.,Ye, Zhengliang.,...&Lin, Ruichao.(2011).An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study.Biomedical chromatography : BMC,25(6),720-6.
MLA Zhou, Dandan,et al."An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study".Biomedical chromatography : BMC 25.6(2011):720-6.

入库方式: OAI收割

来源:上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。