Optimization of Ultrasonic-assisted Extraction of Fatty Acids in Seeds of Brucea Javanica (L.) Merr. from Different Sources and Simultaneous Analysis Using High-Performance Liquid Chromatography with Charged Aerosol Detection
文献类型：期刊论文
| 作者 | Wu, Zhuona2; Li, Ling2; Li, Ning3; Zhang, Tong2; Pu, Yiqiong2; Zhang, Xitong1; Zhang, Yue2; Wang, Bing2 |
| 刊名 | Molecules (Basel, Switzerland)
 |
| 出版日期 | 2017-06-04 |
| 卷号 | 22期号:6 |
| ISSN号 | 1420-3049 |
| DOI | 10.3390/molecules22060931 |
| 文献子类 | Article |
| 英文摘要 | Our research aimed to optimize the oil extraction process and determine the fatty acids in (L.) Merr. seeds. The extraction technology was optimized using response surface methodology. A Box-Behnken design was employed to investigate the effects of three independent variables on an ultrasonic-assisted extraction technique, namely, sonication time (X₁: 20-40 min), liquid-solid ratio (X₂: 16:1 mL/g-24:1 mL/g), and ethanol concentration (X₃: 90%-100%). The optimum conditions of sonication time, liquid-solid ratio, and ethanol concentration were 40 min, 24:1 mL/g, and 100%, respectively. The content of fatty acids and the oil yield were 14.64 mg/g and 16.87%, respectively, which match well with the predicted models. The optimum number of extraction times was eventually identified as two. A new rapid method for the qualitative and quantitative analysis of the fatty acids of (L.) Merr. seed oil using HPLC with a charged aerosol detector was described. The fatty acid contents of 14 batches of (L.) Merr. seed oil were determined, and the relevance and difference were analyzed by fingerprint analysis. The fingerprint has five common peaks, and the similarity was greater than 0.991. HPLC analysis represents a specialized and rational approach for the quality identification and comprehensive evaluation of (L.) Merr. seed oils. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266612]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wu, Zhuona; Li, Ling; Li, Ning; Zhang, Tong; Pu, Yiqiong; Zhang, Xitong; Zhang, Yue; Wang, Bing |
| 作者单位 | 1.Shanghai Xiangshan Hospital of Traditional Chinese Medicine, No. 528, Middle Fuxing Road, Huangpu District, Shanghai 200020, China 2.Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Pudong New District, Shanghai 201203, China; 3.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Pudong New District, Shanghai 201203, China; |
| 推荐引用方式 GB/T 7714 | Wu, Zhuona,Li, Ling,Li, Ning,et al. Optimization of Ultrasonic-assisted Extraction of Fatty Acids in Seeds of Brucea Javanica (L.) Merr. from Different Sources and Simultaneous Analysis Using High-Performance Liquid Chromatography with Charged Aerosol Detection[J]. Molecules (Basel, Switzerland),2017,22(6). |
| APA | Wu, Zhuona.,Li, Ling.,Li, Ning.,Zhang, Tong.,Pu, Yiqiong.,...&Wang, Bing.(2017).Optimization of Ultrasonic-assisted Extraction of Fatty Acids in Seeds of Brucea Javanica (L.) Merr. from Different Sources and Simultaneous Analysis Using High-Performance Liquid Chromatography with Charged Aerosol Detection.Molecules (Basel, Switzerland),22(6). |
| MLA | Wu, Zhuona,et al."Optimization of Ultrasonic-assisted Extraction of Fatty Acids in Seeds of Brucea Javanica (L.) Merr. from Different Sources and Simultaneous Analysis Using High-Performance Liquid Chromatography with Charged Aerosol Detection".Molecules (Basel, Switzerland) 22.6(2017). |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。