Characterization of metabolites of sweroside in rat urine using ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and NMR spectroscopy
文献类型：期刊论文
| 作者 | Han, Han; Zeng, Wenliang; He, Chunyong; Bligh, S W Annie; Liu, Qing; Yang, Li; Wang, Zhengtao |
| 刊名 | Journal of mass spectrometry : JMS
 |
| 出版日期 | 2014-11 |
| 卷号 | 49期号:11页码:1108-16 |
| ISSN号 | 1096-9888 |
| DOI | 10.1002/jms.3429 |
| 文献子类 | Article |
| 英文摘要 | Sweroside, a major active iridoid in Swertia pseudochinensis Hara, is recognized as an effective agent in the treatment of liver injury. Based on previous reports, the relatively short half-life (64 min) and poor bioavailability (approximately 0.31%) in rats suggested that not only sweroside itself but also its metabolites could be responsible for the observed hepato-protective effect. However, few studies have been carried out on the metabolism of sweroside. Therefore, the present study aimed at identifying the metabolites of sweroside in rat urine after a single oral dose (100 mg/kg). With ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC/Q-TOF-MS), the metabolic profile revealed 11 metabolites in rat urine, including phase I, phase II and aglycone-related products. The chemical structures of metabolites were proposed based on accurate mass measurements of protonated or deprotonated molecules and their fragmentation patterns. Our findings showed that the aglycone of sweroside (M05) and its glucuronide conjugate (M06) were principal circulating metabolites in rats. While several other metabolic transformations, occurring via reduction, N-heterocyclization and N-acetylation after deglycosylation, were also observed. Two metabolites (M05 and M06) were isolated from the rat urine for structural elucidation and identifcation of reaction sites. Both M05 and M06 were characterized by (1)H, (13)C and two-dimensional nuclear magnetic resonance (NMR) spectroscopy. UHPLC/Q-TOF-MS analysis has provided an important analytical platform to gather metabolic profile of sweroside. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266614]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines and the State Administration of TCM (SATCM) Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Traditional Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, PR China |
| 推荐引用方式 GB/T 7714 | Han, Han,Zeng, Wenliang,He, Chunyong,et al. Characterization of metabolites of sweroside in rat urine using ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and NMR spectroscopy[J]. Journal of mass spectrometry : JMS,2014,49(11):1108-16. |
| APA | Han, Han.,Zeng, Wenliang.,He, Chunyong.,Bligh, S W Annie.,Liu, Qing.,...&Wang, Zhengtao.(2014).Characterization of metabolites of sweroside in rat urine using ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and NMR spectroscopy.Journal of mass spectrometry : JMS,49(11),1108-16. |
| MLA | Han, Han,et al."Characterization of metabolites of sweroside in rat urine using ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and NMR spectroscopy".Journal of mass spectrometry : JMS 49.11(2014):1108-16. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。