Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour
文献类型：期刊论文
| 作者 | Li, Xiao-Jun4; Bao, Wan-Rong1; Leung, Chung-Hang3; Ma, Dik-Lung2; Zhang, Ge1; Lu, Ai-Ping1; Wang, Shun-Chun4; Han, Quan-Bin1 |
| 刊名 | Molecules (Basel, Switzerland)
 |
| 出版日期 | 2016-06-15 |
| 卷号 | 21期号:6 |
| ISSN号 | 1420-3049 |
| DOI | 10.3390/molecules21060779 |
| 文献子类 | Article |
| 英文摘要 | A neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC) and 1D/2D-NMR spectra data. The backbone of BP1 consists of →₆α-d-Glcp¹→6,3α-d-Glcp¹→(₆α-d-Glcp¹)x-6,3α-d-Glcp¹-(₆α-d-Glcp¹)y→. The side chains were terminal α-d-Glcp¹→ and α-d-Glcp¹→ (₆α-d-Glcp¹)z→₄α-d-Glcp¹→₃α-d-Glcp¹→₄α-d-Glcp¹→ (x + y + z = 5), which are attached to the backbone at O-3 of 3,6α-d-Glcp¹. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266638]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Xiao-Jun; Bao, Wan-Rong; Leung, Chung-Hang; Ma, Dik-Lung; Zhang, Ge; Lu, Ai-Ping; Wang, Shun-Chun; Han, Quan-Bin |
| 作者单位 | 1.School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China; 2.Department of Chemistry, Hong Kong Baptist University, Hong Kong 999077, China 3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China; 4.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; |
| 推荐引用方式 GB/T 7714 | Li, Xiao-Jun,Bao, Wan-Rong,Leung, Chung-Hang,et al. Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour[J]. Molecules (Basel, Switzerland),2016,21(6). |
| APA | Li, Xiao-Jun.,Bao, Wan-Rong.,Leung, Chung-Hang.,Ma, Dik-Lung.,Zhang, Ge.,...&Han, Quan-Bin.(2016).Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour.Molecules (Basel, Switzerland),21(6). |
| MLA | Li, Xiao-Jun,et al."Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour".Molecules (Basel, Switzerland) 21.6(2016). |
入库方式： OAI收割
来源：上海药物研究所
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