Content decline of SERCA inhibitors saikosaponin a and d attenuates cardiotoxicity and hepatotoxicity of vinegar-baked Radix bupleuri
文献类型：期刊论文
| 作者 | Wang, Shifeng4; Zhang, Yuxin4; Zhang, Qiao4; Peng, Sha4; Shen, Chen4; Yu, Yangyang4; Zhang, Minyu1; Yang, Wei5; Wu, Qinghua2; Zhang, Yanling4 |
| 刊名 | Environmental toxicology and pharmacology
 |
| 出版日期 | 2017-06 |
| 卷号 | 52页码:129-137 |
| ISSN号 | 1872-7077 |
| DOI | 10.1016/j.etap.2017.04.002 |
| 文献子类 | Article |
| 英文摘要 | Improper usage of unprocessed Radix bupleuri root (chaihu) may cause cardiotoxicity and liver injury. Baking herb with vinegar is believed to attenuate the adverse responses. However, the chemical and molecular basis involved remained unclear. To this end, we investigated the in vitro toxicity of saikosaponin a, c, d, and their hydrolysates saikosaponin b1 and b2. Results showed that SSa and SSd possessed higher affinity with sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) by molecular docking, and exhibited stronger toxic responses on cardiomyocytes and hepatocytes than the other three saikosaponins in equivalent concentrations. Further, SSa and SSd induced LC3 puncta formation in U2OS-mCherry-EGFP-LC3 cells. Blockage of autophagy by 3-methyladenine did not abrogate the cytotoxicities induced by SSa and SSd. In parallel, none of SSc, SSb1, or SSb2 caused cell injury. Our study reveals how changes in chemical ingredients are connected to the toxicity of Chaihu during vinegar baking process and also provides a guidance for structure optimization to reduce drug induced toxicity. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266676]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Shiyou; Qiao, Yanjiang |
| 作者单位 | 1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 2.HD Biosciences Co., Ltd, Shanghai 201201, China; 3.Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China 4.School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China; 5.ACEA Biosciences Inc., Hangzhou 310030, China; |
| 推荐引用方式 GB/T 7714 | Wang, Shifeng,Zhang, Yuxin,Zhang, Qiao,et al. Content decline of SERCA inhibitors saikosaponin a and d attenuates cardiotoxicity and hepatotoxicity of vinegar-baked Radix bupleuri[J]. Environmental toxicology and pharmacology,2017,52:129-137. |
| APA | Wang, Shifeng.,Zhang, Yuxin.,Zhang, Qiao.,Peng, Sha.,Shen, Chen.,...&Qiao, Yanjiang.(2017).Content decline of SERCA inhibitors saikosaponin a and d attenuates cardiotoxicity and hepatotoxicity of vinegar-baked Radix bupleuri.Environmental toxicology and pharmacology,52,129-137. |
| MLA | Wang, Shifeng,et al."Content decline of SERCA inhibitors saikosaponin a and d attenuates cardiotoxicity and hepatotoxicity of vinegar-baked Radix bupleuri".Environmental toxicology and pharmacology 52(2017):129-137. |
入库方式： OAI收割
来源：上海药物研究所
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