An integrated approach to identify critical transcription factors in the protection against hydrogen peroxide-induced oxidative stress by Danhong injection
文献类型：期刊论文
| 作者 | Zhang, Jingjing2; Guo, Feifei2; Wei, Junying2; Xian, Minghua2; Tang, Shihuan2; Zhao, Ye2; Liu, Mingwei3; Song, Lei3; Geng, Ya4; Yang, Hongjun2 |
| 刊名 | Free radical biology & medicine
 |
| 出版日期 | 2017-11 |
| 卷号 | 112页码:480-493 |
| ISSN号 | 1873-4596 |
| DOI | 10.1016/j.freeradbiomed.2017.07.002 |
| 文献子类 | Article |
| 英文摘要 | Oxidative stress plays a vital role in many pathological processes of the cardiovascular diseases. However, the underlying mechanism remains unclear, especially on a transcription factor (TF) level. In this study, a new method, concatenated tandem array of consensus transcription factor response elements (catTFREs), and an Illumina-based RNA-seq technology were integrated to systematically investigate the role of TFs in hydrogen peroxide (HO)-induced oxidative stress in cardiomyocytes; the damage was then rescued by Danhong injection (DHI), a Chinese standardized product approved for cardiovascular diseases treatment. The overall gene expression revealed cell apoptosis and DNA repair were vital for cardiomyocytes in resisting oxidative stress. By comprehensively integrating the transcription activity of TFs and their downstream target genes, an important TFs-target network were constructed and 13 TFs were identified as critical TFs in DHI-mediated protection in HO-induced oxidative stress. By using the integrated approach, seven TFs of these 13 TFs were also identified in melatonin-mediated protection in HO-induced damage. Furthermore, the transcription activity of DNA-(apurinic or apyrimidinic site) lyase (Apex1), Myocyte-specific enhancer factor 2D (Mef2d) and Pre B-cell leukemia transcription factor 3 (Pbx3) was further verified in pluripotent stem cell-derived cardiomyocytes. This research offers a new understanding of cardiomyocytes in response to HO-induced oxidative stress and reveals additional potential therapeutic targets. The combination of two parallel omics datasets (corresponding to the transcriptome and proteome) can reduce the noise in high-throughput data and reveal the fundamental changes of the biological process, making it suitable and reliable for investigation of critical targets in many other complicated pathological processes. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266771]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Hongjun; Ding, Chen; Huang, Luqi |
| 作者单位 | 1.State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Institute of Biomedical Sciences, Fudan University, Shanghai 200433, China; 2.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 3.State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; 4.School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 5.State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| 推荐引用方式 GB/T 7714 | Zhang, Jingjing,Guo, Feifei,Wei, Junying,et al. An integrated approach to identify critical transcription factors in the protection against hydrogen peroxide-induced oxidative stress by Danhong injection[J]. Free radical biology & medicine,2017,112:480-493. |
| APA | Zhang, Jingjing.,Guo, Feifei.,Wei, Junying.,Xian, Minghua.,Tang, Shihuan.,...&Huang, Luqi.(2017).An integrated approach to identify critical transcription factors in the protection against hydrogen peroxide-induced oxidative stress by Danhong injection.Free radical biology & medicine,112,480-493. |
| MLA | Zhang, Jingjing,et al."An integrated approach to identify critical transcription factors in the protection against hydrogen peroxide-induced oxidative stress by Danhong injection".Free radical biology & medicine 112(2017):480-493. |
入库方式： OAI收割
来源：上海药物研究所
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