Investigating the relationship between CD20-Rituximab binding force and mechanical properties of Lymphom B cells using atomic force microscopy
文献类型：会议论文
| 作者 | Li, Mi2,4,5; Liu, Lianqing2; Xi, Ning1,2; Wang, Yuechao2; Dong, Zaili2; Xiao, Xiubin3; Zhang, Weijing3 |
| 出版日期 | 2012 |
| DOI | 10.1109/3M-NANO.2012.6472926 |
| 页码 | 50-53 |
| 英文摘要 | The invention of atomic force microscopy (AFM) provides new technology for investigating the physiological activities at the single cell and single molecule levels. Lymphoma Raji cells were adsorbed onto the glass slides by coating the glass slides with poly-L-lysine. Rituximabs (anti-CD20 antibody) were linked onto the AFM tip by PEG linker and the CD20-Rituximab binding forces were measured on three lymphoma Raji cells. Then the mechanical properties of the Raji cells were dynamically measured after the Rituximab activation by obtaining force curves on the cell surface. The mechanical properties of lymphoma cells kept stable at the first 20-30 min, and then decreased markedly. Besides, as the CD20-Rituximab binding force increased, the mechanical properties decrease rate increased. The experimental results indicated that the Rituximab's effect is related to CD20-Rituximab binding force and can improve our understanding of Rituximab's variable efficacies in different patients. |
| 会议录 | 2012 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2012 - Conference Proceedings
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| 会议录出版者 | IEEE Computer Society |
| 文献子类 | Proceedings Paper |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266854]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Mi |
| 作者单位 | 1.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, United States; 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China; 3.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China 4.Graduate University of Chinese Academy of Sciences, Beijing, China; 5.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; |
| 推荐引用方式 GB/T 7714 | Li, Mi,Liu, Lianqing,Xi, Ning,et al. Investigating the relationship between CD20-Rituximab binding force and mechanical properties of Lymphom B cells using atomic force microscopy[C]. 见:. |
入库方式： OAI收割
来源：上海药物研究所
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