Imaging and measuring the protein distribution of lymphoma cells using atomic force microscopy
文献类型：会议论文
| 作者 | Li, Mi1,3,4; Liu, Lianqing3; Xi, Ning3,5; Wang, Yuechao3; Dong, Zaili3; Li, Guangyong3,6; Tabata, Osamu7; Xiao, Xiubin2; Zhang, Weijing2 |
| 出版日期 | 2011 |
| DOI | 10.1109/NEMS.2011.6017326 |
| 页码 | 188-191 |
| 英文摘要 | Atomic force microscopy (AFM) has proven to be a powerful tool for imaging the topographic properties of biological samples and measuring the molecular specific binding forces at cellular and molecular levels. However, there are still great difficulties in using AFM to acquire images of mammalian suspension live cells due to the suspension growth manner and the softness of the cell membrane. In this paper, lymphoma live cells were trapped in microfabricated patterned pillars and then imaged by AFM in phosphate buffered saline (PBS) solution. In addition, the CD20 distribution on the lymphoma cell surface was visualized three-dimensionally by tethering rituximabs (anti-CD20 monoclonal antibody) onto the AFM tips. The experimental results indicate that immobilization with microfabricated patterned pillars provides a novel way for anchoring suspension mammalian cells, opening the way for further investigation of the molecular mechanisms of rituximab's anti-cancer effect. |
| 会议录 | NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
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| 会议录出版者 | IEEE Computer Society |
| 文献子类 | Proceedings Paper |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266860]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Lianqing |
| 作者单位 | 1.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, 201203, China 2.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, 100071, China; 3.State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shenyang, 110016, China; 4.Graduate University of Chinese Academy of Sciences, Beijing, 100049, China; 5.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, United States; 6.Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA,15261, United States; 7.Department of Micro Engineering, Kyoto University, Kyoto, 606-8501, Japan; |
| 推荐引用方式 GB/T 7714 | Li, Mi,Liu, Lianqing,Xi, Ning,et al. Imaging and measuring the protein distribution of lymphoma cells using atomic force microscopy[C]. 见:. |
入库方式： OAI收割
来源：上海药物研究所
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