Studies on polysaccharide peptide with high performance gel permeation chromatography
文献类型：会议论文
| 作者 | Zhang, Z; Yang, X; Fang, J |
| 出版日期 | 1997-03 |
| 卷号 | 15 |
| 期号 | 2 |
| 页码 | 150-2 |
| 英文摘要 | High performance gel permeation chromatography (HPGPC) has been widely used in the fraction and molecular-weight (MW) estimation of polysaccharides. In this paper, we estimated the MW of major component of polysaccharide-peptide from Corilous versicolor (PSP). These three-components were separated by using two column system (TSK-40 and TSK-50 gel columns) and different NaCl concentration in mobile phase. Calibration curve was prepared from the known MW Dextran T-system. It was found that the MW of PSP was changed in accordance with ion concentration of mobile phase. The MWs of the main component are more than 500000, 120000-19000 and less than 10000 respectively as the NaCl concentrations in mobile phase were less than 0.002, 0.006-0.04 and more than 0.06mol/L. The results showed that the MW of polysaccharide-peptide determined by HPGPC is a relative value that is changed with the concentration of mobile phase. The reason is that the molecular configuration and size of polysaccharide-peptide are changed along with electrolyte concentration in mobile phase. That is, the lower the electrolyte concentration is, the larger the molecular size of polysaccharide-peptide is. We suggest that it is still useful for quality control of PSP as the mobile phase is fixed. |
| 会议录 | Se pu = Chinese journal of chromatography
 |
| 文献子类 | Meeting Abstract;Article |
| 语种 | 中文 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266939]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Shanghai Institute of Materia Medica, the Chinese Academy of Sciences, Shanghai, 200031 |
| 推荐引用方式 GB/T 7714 | Zhang, Z,Yang, X,Fang, J. Studies on polysaccharide peptide with high performance gel permeation chromatography[C]. 见:. |
入库方式： OAI收割
来源：上海药物研究所
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