Coordinated biosynthesis of the purine nucleoside antibiotics aristeromycin and coformycin in actinomycetes
文献类型：期刊论文
| 作者 | Xu, Gudan1; Kong, Liyuan1,2; Gong, Rong1; Xu, Liudong3; Gao, Yaojie3; Jiang, Ming3; Cai, You-Sheng1; Hong, Kui1; Hu, Youcai2; Liu, Peng1 |
| 刊名 | Applied and Environmental Microbiology
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 84期号:22 |
| ISSN号 | 00992240 |
| DOI | 10.1128/AEM.01860-18 |
| 文献子类 | Article |
| 英文摘要 | Purine nucleoside antibiotic pairs, concomitantly produced by a single strain, are an important group of microbial natural products. Here, we report a target-directed genome mining approach to elucidate the biosynthesis of the purine nucleoside antibiotic pair aristeromycin (ARM) and coformycin (COF) in Micromonospora haikouensis DSM 45626 (a new producer for ARM and COF) and Streptomyces citricolor NBRC 13005 (a new COF producer). We also provide biochemical data that MacI and MacT function as unusual phosphorylases, catalyzing an irreversible reaction for the tailoring assembly of neplanocin A (NEP-A) and ARM. Moreover, we demonstrate that MacQ is shown to be an adenosine-specific deaminase, likely relieving the potential "excess adenosine" for producing cells. Finally, we report that MacR, an annotated IMP dehydrogenase, is actually an NADPH-dependent GMP reductase, which potentially plays a salvage role for the efficient supply of the precursor pool. Hence, these findings illustrate a fine-tuned pathway for the biosynthesis of ARM and also open the way for the rational search for purine antibiotic pairs. IMPORTANCE ARM and COF are well known for their prominent biological activities and unusual chemical structures; however, the logic of their biosynthesis has long been poorly understood. Actually, the new insights into the ARM and COF pathway will not only enrich the biochemical repertoire for interesting enzymatic reactions but may also lay a solid foundation for the combinatorial biosynthesis of this group of antibiotics via a target-directed genome mining strategy. |
| 语种 | 英语 |
| 出版者 | American Society for Microbiology |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266965]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Wenqing |
| 作者单位 | 1.Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China; 2.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; 3.State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China; 4.Agricultural Research Service, U.S. Department of Agriculture, National Center for Agricultural Utilization Research, Peoria; IL, United States |
| 推荐引用方式 GB/T 7714 | Xu, Gudan,Kong, Liyuan,Gong, Rong,et al. Coordinated biosynthesis of the purine nucleoside antibiotics aristeromycin and coformycin in actinomycetes[J]. Applied and Environmental Microbiology,2018,84(22). |
| APA | Xu, Gudan.,Kong, Liyuan.,Gong, Rong.,Xu, Liudong.,Gao, Yaojie.,...&Chen, Wenqing.(2018).Coordinated biosynthesis of the purine nucleoside antibiotics aristeromycin and coformycin in actinomycetes.Applied and Environmental Microbiology,84(22). |
| MLA | Xu, Gudan,et al."Coordinated biosynthesis of the purine nucleoside antibiotics aristeromycin and coformycin in actinomycetes".Applied and Environmental Microbiology 84.22(2018). |
入库方式： OAI收割
来源：上海药物研究所
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