Heterologous expression of human paraoxonases in Pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance
文献类型：期刊论文
| 作者 | Ma, Fang1,2,3; Wang, Yao3; Zhang, Yong1,3; Xiong, Ning1,3; Yang, Baoyu3; Chen, Shiyun3 |
| 刊名 | Applied Microbiology and Biotechnology
 |
| 出版日期 | 2009-05 |
| 卷号 | 83期号:1页码:135-141 |
| ISSN号 | 01757598 |
| DOI | 10.1007/s00253-009-1890-2 |
| 文献子类 | Article |
| 英文摘要 | Quorum sensing (QS) regulates virulence and biofilm formation in Pseudomonas aeruginosa and other medically relevant bacteria. Human paraoxonases (hPONs) are a family of closely related enzymes with multiple functions, including inactivation of the QS signal molecule in P. aeruginosa. However, there is no direct evidence to show the functions of hPONs on biofilm formation and antibiotic resistance in P. aeruginosa. In the present study, hPONs (hPON1, hPON2, and hPON3) genes were respectively cloned into the pMEKm12 shuttle vector and transformed into P. aeruginosa strain PAO1. Expression of the three recombinant proteins was confirmed by Western blotting, and growth of the recombinant strains was not affected by the hPONs gene expression. Biofilm formation and antibiotics resistance of the hPONs recombinant strains were analyzed. Our results showed that biofilm formation was significantly inhibited in all of the three hPONs recombinant strains. Interestingly, this inhibition can be reverted by addition of the corresponding hPONs polyclonal antibodies in the culture media, further indicating that the inhibition of biofilm formation was due to hPONs protein expression. In addition, we also demonstrated that hPONs expression decreased resistance of P. aeruginosa to gentamicin and ceftazidima, two antibiotics clinically used for the treatment of P. aeruginosa infection. |
| 语种 | 英语 |
| 出版者 | Springer Verlag |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266996]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Graduate School, Chinese Academy of Sciences, Beijing 100049, China; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China 3.Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei Province 430071, China; |
| 推荐引用方式 GB/T 7714 | Ma, Fang,Wang, Yao,Zhang, Yong,et al. Heterologous expression of human paraoxonases in Pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance[J]. Applied Microbiology and Biotechnology,2009,83(1):135-141. |
| APA | Ma, Fang,Wang, Yao,Zhang, Yong,Xiong, Ning,Yang, Baoyu,&Chen, Shiyun.(2009).Heterologous expression of human paraoxonases in Pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance.Applied Microbiology and Biotechnology,83(1),135-141. |
| MLA | Ma, Fang,et al."Heterologous expression of human paraoxonases in Pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance".Applied Microbiology and Biotechnology 83.1(2009):135-141. |
入库方式： OAI收割
来源：上海药物研究所
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