Effect of Pinellia Extract on HeLa Cell line In vitro and Associated Mechanism
文献类型：期刊论文
| 作者 | LI Gui-ling; GUI Sui-qi; Wang Li |
| 刊名 | Chinese Journal of Integrated Traditional and Western Medicine
 |
| 出版日期 | 2010 |
| 卷号 | 30期号:3页码:303-307 |
| 关键词 | Pinellia extract cervical cancer HeLa cell line proliferating cell nuclear antigen B cell lymphoma/leukemia-2 |
| ISSN号 | 1003-5370 |
| 其他题名 | 掌叶半夏有效提取物对HeLa细胞的作用及机制 |
| 文献子类 | Article |
| 英文摘要 | Objective To investigate the effect of Pinellia extract (PE) on HeLa cell line and to study its associated mechanisms,in order to provide theoretical foundations for its applying in clinical prevention and treatment of cervical disease.Methods HeLa cell line was incubated in media containing different concentrations of PE.Growth of cells was observed and photo-generated with inverted phase microscope;cell activity was detected by MTT assay;cell apoptosis detected by flow cytometry,protein expression of proliferating cell nuclear antigen proliferating cell nuclear antigen (PCNA) was detected by immuno-cytochemistry,and protein expression of Bcl-2 was determined by immunofluorescence.Results PE showed obvious inhibition on the proliferation of HeLa cells,cell apoptosis appeared after PE treatment in a time and dose dependent manner;PCNA and Bcl-2 protein expressions reduced significantly after being effected by PE for 24 h.Conclusions PE can obviously inhibit the growth and induce the apoptosis of HeLa cells,and its mechanism is possibly realized through down-regulating the expressions of PCNA and Bcl-2.The study set a primary experimental base for further studying the action mechanism of PE and developing new anti-tumor agents. |
| 资助项目 | 上海市科委科研计划项目[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3858640 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267917]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Department of Integrated Traditional and Western Medicine,Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China. |
| 推荐引用方式 GB/T 7714 | LI Gui-ling,GUI Sui-qi,Wang Li. Effect of Pinellia Extract on HeLa Cell line In vitro and Associated Mechanism[J]. Chinese Journal of Integrated Traditional and Western Medicine,2010,30(3):303-307. |
| APA | LI Gui-ling,GUI Sui-qi,&Wang Li.(2010).Effect of Pinellia Extract on HeLa Cell line In vitro and Associated Mechanism.Chinese Journal of Integrated Traditional and Western Medicine,30(3),303-307. |
| MLA | LI Gui-ling,et al."Effect of Pinellia Extract on HeLa Cell line In vitro and Associated Mechanism".Chinese Journal of Integrated Traditional and Western Medicine 30.3(2010):303-307. |
入库方式： OAI收割
来源：上海药物研究所
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