Molecular Conformation and Biological Activity of Truncated Cell Toxin, Gelonin
文献类型：期刊论文
| 作者 | QU YanFeng2; LI ZhuoYu2; GUO ChenYun3; GUO SuTang1; SHI TianLiang1; YUAN JingMing2 |
| 刊名 | Chinese Journal of Biochemistry and Molecular Biology
 |
| 出版日期 | 2006 |
| 卷号 | 22期号:5页码:390-395 |
| 关键词 | truncated gelonin purification conformation DNase-like activity inhibiting tumour cells |
| ISSN号 | 1007-7626 |
| 其他题名 | 截短型细胞毒素Gelonin的分子构象和生物活性 |
| 文献子类 | Article |
| 英文摘要 | To explore the relationship between the structure and function of cell toxin gelonin as the bullet of immunoconjugate to specially kill and wound target cells, truncated gelonins deleted at N-terminal region Gly, Leu, Asp and/or C-terminal Asp, Lys, Asp, Pro, Lys were designed on the basis of its gene sequence and three dimentional molecular conformation. DNA fragments of recombinant gelonin and its truncated products were amplified by PCR with plasmid pE-gel as the template in the presence of different primers, and in turn, the relevant proteins, e.g. intact gelonin （G-O） and truncated gelonins （G-N3, G-C5, G-N3C5） were obtained by processes of cloning, expression and purification. CD and fluorescence spectra evaluating the conformational change of protein show that truncated gelonins are obviously different from the intact molecule, showing that the alpha-helix relative content in truncated form decreased. Furthermore, DNase-like activity in vitro and the ability of inhibiting tumour cell growth in vivo are as following G-O≥G-N3〉G-C5〉G-N3C5. In conclusion, results in this study demonstrate the consistency of the structure and bioactivity of gelonin with the protein of alpha ＋ beta conformation. |
| 资助项目 | 国家自然科学基金资助项目[00000000] |
| WOS研究方向 | Genetics & Heredity ; Biochemistry & Molecular Biology (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2463715 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268195]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Shanxi Institute of Cancer Research, Taiyuan, Shanxi 030013, China. 2.Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi 030006, China.; 3.Shanghai Institute of Meteria Medica, Chinese Academy of Sciences, Shanghai 201203, China.; |
| 推荐引用方式 GB/T 7714 | QU YanFeng,LI ZhuoYu,GUO ChenYun,et al. Molecular Conformation and Biological Activity of Truncated Cell Toxin, Gelonin[J]. Chinese Journal of Biochemistry and Molecular Biology,2006,22(5):390-395. |
| APA | QU YanFeng,LI ZhuoYu,GUO ChenYun,GUO SuTang,SHI TianLiang,&YUAN JingMing.(2006).Molecular Conformation and Biological Activity of Truncated Cell Toxin, Gelonin.Chinese Journal of Biochemistry and Molecular Biology,22(5),390-395. |
| MLA | QU YanFeng,et al."Molecular Conformation and Biological Activity of Truncated Cell Toxin, Gelonin".Chinese Journal of Biochemistry and Molecular Biology 22.5(2006):390-395. |
入库方式： OAI收割
来源：上海药物研究所
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