Identification of inhibitors that target JAK-STAT6 signal pathway via high-throughput screening
文献类型：期刊论文
| 作者 | DA Yurong1; YAO Zhi1; LI Jingya2 ; SHAO Jie1; SHEN Qiang2; DONG Lijie1; LI Jia2; YANG Jie1
|
| 刊名 | Chinese Pharmacological Bulletin
 |
| 出版日期 | 2006 |
| 卷号 | 22期号:8页码:943-946 |
| 关键词 | JAK/STAT6 JAK/STAT6 high throughput screen cell model inhibitor |
| ISSN号 | 1001-1978 |
| 其他题名 | 高通量筛选JAK-STAT6信号传导通路抑制剂方法的初步建立 |
| 文献子类 | Article |
| 英文摘要 | To establish the stable cell line which expresses luciferase depending on STAT6-raediated gene transcriptional activation. The cell line is used as a model to screen compounds that inhibit JAK- STAT6 signal transduction pathway. Methods The sequence of IgE promoter and luciferase reporter gene were sub-cloned into the vector to generate recombinant plasmid, which can express luciferase after IL-4- stimulation. The recombinant plasmid was cotransfected into HeLa cells together with hygromycin B DNA. The positive cell clones were selected in the culture media which contains hygromycin B. Some factors such as final concentration of DMSO and working concentration of IL-4 were measured to optimize the assay condition. 1600 compounds were screened by the method. Results A JAK-STAT6 activation depended cell model was setup and a reliable method was established to perform high throughput screening. Z'-factor value of the system was near 0. 64. Three compounds were screened to have suppressive effect on JAK-STAT6 pathway. Conclu- sion This assay can be applied to identify inhibitors targeting JAK-STAT6 signal transduction pathway. |
| 资助项目 | 国家自然科学基金资助项目[00000000] ; 国家教育部新世纪人才支持计划项目[00000000] ; 高等学校博士学科点专项科研基金资助项目[00000000] ; 天津市应用基础研究重点资助项目[00000000] ; 天津医科大学自然科学基金资助项目[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2432179 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268212]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Depl of Immunology, Tianjin Medical University, Tianjin 300070, China.; 2.National Center for Drug Screening, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | DA Yurong,YAO Zhi,LI Jingya,et al. Identification of inhibitors that target JAK-STAT6 signal pathway via high-throughput screening[J]. Chinese Pharmacological Bulletin,2006,22(8):943-946. |
| APA | DA Yurong.,YAO Zhi.,LI Jingya.,SHAO Jie.,SHEN Qiang.,...&YANG Jie.(2006).Identification of inhibitors that target JAK-STAT6 signal pathway via high-throughput screening.Chinese Pharmacological Bulletin,22(8),943-946. |
| MLA | DA Yurong,et al."Identification of inhibitors that target JAK-STAT6 signal pathway via high-throughput screening".Chinese Pharmacological Bulletin 22.8(2006):943-946. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。