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Establishment of heparanase stable expression cell lines

文献类型：期刊论文

作者QU Xiaoting2; ZHAO Huajun1; ZHANG Zhonghua1; HOU Yongtai1; XI Tao2
刊名Journal of China Pharmaceutical University
出版日期2006
卷号37期号:1页码:72-76
关键词heparanase tumor metastasis stable expression
ISSN号1000-5048
其他题名乙酰肝素酶稳定表达细胞株的建立
文献子类Article
英文摘要Aim： To express human heparanase in eucaryote cells and establish stable cell lines. Methods： cDNA encoding the open reading frame of human heparanase was inserted into pcDNA3.1/myc-His C. This plasmid was then transfected into CHO-K1 before stable cell lines were established by screening with CA18. RT-PCR and Western-blot were applied to detect its expression;flurescent HPLC was used to evaluate its activity. Results：The CHO-K1 cells transfected with pcDNA3.1/myc-His C produced active heparanse protein, and the stable cell lines were confirm to be established. Conclusion：Heparanase stable expression cell lines have been successfully established, which could be of great benefits to the screening and function study of its inhibitors.
资助项目2001年中国科学院百人计划资助项目[00000000]
WOS研究方向Genetics & Heredity (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:2314857
源URL[http://119.78.100.183/handle/2S10ELR8/268217]  
专题中国科学院上海药物研究所
作者单位1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
2.School of Life Science ＆ Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, China.;
推荐引用方式
GB/T 7714		 QU Xiaoting,ZHAO Huajun,ZHANG Zhonghua,et al. Establishment of heparanase stable expression cell lines[J]. Journal of China Pharmaceutical University,2006,37(1):72-76.
APA QU Xiaoting,ZHAO Huajun,ZHANG Zhonghua,HOU Yongtai,&XI Tao.(2006).Establishment of heparanase stable expression cell lines.Journal of China Pharmaceutical University,37(1),72-76.
MLA QU Xiaoting,et al."Establishment of heparanase stable expression cell lines".Journal of China Pharmaceutical University 37.1(2006):72-76.

入库方式： OAI收割

来源：上海药物研究所

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